Four more adhesion experiments were carried out and the results can be seen in the following charts. Based on the average of the four trials it can be elucidated that the initial adhesion (after ~7 hours) is pretty much the same in all the substrates. In trial 3 & 4 it can be seen that…
Two more adhesion tests were run recently, one on Tuesday and one on Wednesday. For both tests cells were imaged after five hours. Fibroblast trial 3 was the first test where the optimal cell density (assuming 100% of cells are detached by trypsin and 100% attach to the substrate) was measured using a hemacytometer. Of…
Thus far three HUVEC and two L13T3 growth tests have been conducted on substrates including glass,pnc-Si, tissue culture plastic, and untreated plastic. Only one test with untreated plastic has been run for each cell type. Cells on Si samples are not on the slits but rather on substrate surrounding. It seems that HUVEC proliferate more…
Adhesions tests are being performed to see if cellular attachment varies between our material, glass, and plastic. These experiments are motivated by lower initial cell densities observed on pnc-Si in growth tests; we are investigating if the lower initial densities are due to the cells rolling of the edge of the raised pnc-Si substrate or…
HUVEC (P5) growth was monitored over 3 days on different substrates (glass coverslips, tissue culture treated & untreated plastic and RTPed pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate on pncSi is higher followed by glass. As expected, the growth on treated plastic is…
A third fibroblast (L1 3T3, P22) growth test was conducted using glass, untreated and tissue culture treated plastic, and RTPed pnc-Si (W509) as substrates. Cells were grown in DMEM +10% FBS changed every 48 hours. R values are for the fit N=No*exp^(r*t), where r has units of divisions/hour. For this trial fibroblasts grown on glass,…
HUVEC (P8) growth was monitored over 5 days on different substrates (glass coverslips, tissue culture treated plastic and pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate on pncSi in two different trials were observed to be pretty much the same. Decrease in the growth…
A second fibroblast (L1 3T3, P21) growth test was conducted on RTP treated samples from W510, tissue culture plastic (polystyrene), and glass cover slips. Fibroblasts were cultured in DMEM with media changed every 48 hours. Cells were imaged using the live/dead assay. Fibroblasts divided every 29.9, 35, and 37.7 hours when grown on pnc-Si, glass,…
Last week I attempted another HUVEC growth study on Si, coverslips (glass), and culture wells (plastic). The cells were seeded at too low of a density on the membrane so I adjusted my protocol and started a third growth test. The results of the third growth experiment is shown below. After 86 hours the cells…
(Transferred from old blog) From the above discoloration array it can be inferred that the RTP treated samples in the incubator are discoloring in much slower rate than the ones on the benchtop. Thus, incubator conditions prevent the chips from discoloring. The reason behind this could be the carbon dioxide present in th incubator which…