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Nanomembrane Research Group
  • NRG

    MgF2 + Si3N4 Nanomembrane Images

    ByGreg Madejski December 28, 2013January 15, 2014

    Here are some images of deposited magnesium fluoride films on 50 nanonitride:              

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  • NRG

    Sepcon Documentation

    ByKarl Smith December 23, 2013

    On Friday, Henry was kind enough to ship 15 tested Sepcons to the Barcikowski group in Essen, Germany. With luck they will arrive in time for Christmas. I wrote up some documentation and an illustration for wetting and using the Sepcons and figured I would share it with the group. Unfortunately, wordpress won’t let me…

    Read More Sepcon DocumentationContinue

  • NRG

    72 Hour Diffusion Test Results

    ByTucker Burgin December 22, 2013July 7, 2014

    I recently got some good looking results on the FLAG diffusion test over a 24 hour period. The relative concentrations of enzyme seemed to indicate that measurably more enzyme diffused from retentate to filtrate in solutions not containing constricting Anti-FLAG antibody, and this is excellent news, but over the 24 hours between beginning the diffusion…

    Read More 72 Hour Diffusion Test ResultsContinue

  • NRG

    The effects of driving pressure on sieving behavior in SiN Sepcons

    ByKarl Smith December 18, 2013July 7, 2014

    We’ve been wondering for some time just how the thinness of our membrane will affect the concentration polarization behavior of solutes. Because solutes with sieving coefficients less than 1 are (by definition) moving slower than the solvent around them, in the area before the membrane their concentration increases. In the case of proteins, this so-called…

    Read More The effects of driving pressure on sieving behavior in SiN SepconsContinue

  • NRG

    Subscripts and Superscripts

    ByDean Johnson December 18, 2013

    Helpful Hint: H<sub>2</sub>O typed as text will yield H2O visually, and E = mc<sup>2</sup>O typed as text will yield E = mc2. Of course this affects line height, so choose your poison, I guess.

    Read More Subscripts and SuperscriptsContinue

  • NRG

    Assembly statistics for SiN wafers 1070 and 1081

    ByKarl Smith December 17, 2013

    I’ve been on a Sepcon assembly binge, and I thought I would post my assembly statistics. On 12/15/13 (assembly “L”) I broke 28 chips out of wafer 1070. 3 chips were visually damaged by the break-out process, 1 was already defective. After inserting the chips into the sepcon housing, testing with 5 psi nitrogen revealed…

    Read More Assembly statistics for SiN wafers 1070 and 1081Continue

  • NRG

    Interesting Paper: Bioengineered Rat Kidney

    ByDean Johnson December 13, 2013

    Earlier this year an MIT/Harvard group generated and transplanted a rat kidney and published in Nature Medicine Technical Report. “Regeneration and experimental orthotopic transplantation of a bioengineered kidney” Jeremy J Song, Jacques P Guyette, Sarah E Gilpin, Gabriel Gonzalez, Joseph P Vacanti, & Harald C Ott Center for Regenerative Medicine, Massachusetts General Hospital and Harvard…

    Read More Interesting Paper: Bioengineered Rat KidneyContinue

  • NRG

    Nanoporous Nitride Membranes: 400-µm wide channels, a pictorial survey

    ByDean Johnson December 13, 2013

    Images of the nanoporous nitride wafer. The same wafer that Josh declared as having 30% porosity with an average pore size of 80 nm (or something similar). Josh put images and such on basecamp for those who have access. These numbers are for a TEM grid from near the outside edge of the wafer, where…

    Read More Nanoporous Nitride Membranes: 400-µm wide channels, a pictorial surveyContinue

  • Protocols

    Small Animal Model Hemodialysis Set-Up

    ByDean Johnson December 12, 2013July 7, 2014

    Sprague-Dawley rats will be purchased from Harlan. These pre-manipulated animals will have been catheterized prior to shipment. We will feed them an adenine rich diet to induce uremia, high levels of urea in the blood. Once uremic, a rat will be connected via blunted needles to the dialysis system. Heparin, or other substances, may be…

    Read More Small Animal Model Hemodialysis Set-UpContinue

  • NRG

    Organic-Inorganic Hybrid Membranes Formed from Block Copolymers and Titanium Dioxide Nanoparticles (A Review)

    ByKarl Smith December 11, 2013

    The Wiesner lab at Cornell (whom you may remember as the discoverer of the neutrally charged but highly fluorescent “C-dots” Jess used for her charged-based separation work) are about to publish Asymmetric Organic-Inorganic Hybrid Membrane Formation via Block Copolymer-Nanoparticle Co-Assembly in Nano letters. Building upon their previous work on block copolymer self-assembling films, they have added titanium dioxide nanoparticles…

    Read More Organic-Inorganic Hybrid Membranes Formed from Block Copolymers and Titanium Dioxide Nanoparticles (A Review)Continue

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    • Home
    • Publications
    • Membranes
      • Common Chip Formats
      • Common Membranes
      • Microslit Membranes
    • Devices
      • µSiM
        • Geometry
        • µSiM CAD Files
        • Assembly
          • Protocols.io (µSiM Assembly)
          • Instructions
          • Common Issues and Troubleshooting Tips
        • Cell Culture Protocols
          • Top Well: hCMEC/D3
          • Top Well: HUVEC
          • Bottom Channel Culturing
          • Immunocytochemistry Protocol
          • Impact of Chip Orientation on Fluorescence Imaging
          • Permeability: In Situ Method
          • Permeability: Sampling Method
          • Cell Culture Common Issues and Troubleshooting Tips
      • SepCon®
        • Sepcon Assembly
        • Sepcon Video Protocol: Assembly
        • SepCon Gasket Silhouette File
        • SepCon Video Protocol: Wetting the membrane
        • SepCon Video Protocol: Disassembly
      • µSiM-DX
        • µSIM Video Protocol: Capture of Nanoparticles
    • Impact
      • TraCe-bMPS
      • HCIC
      • LOMP
      • SiMPore