For context, see my previous post. Early on in the process of designing an experiment to confirm the usefulness of a CytoVu assembly for use as an assay, it was decided that the best solution for measuring the extremely low concentrations [ng/mL scale] of bacterial alkaline phosphatase (“bAP”) in the filtrate which would indicate what…
My streaming potential device is now working consistently. It turns out that the problem was the concentrations of salt that I was using. The Debye Length (the distance over which charge-charge interactions are important in an electrolyte) is given as , where is the debye length in nm and is the molar concentration of…
In working on a long over due paper by Barrett on endothelial response to high permeability material … Key figs … Figure 2 Figure 4 Figure 5 And one post from Nakul. I came across this highly relevant paper Pedrigi(Overby)EER11
In vitro Clearance and Hemocompatibility Assessment of Ultrathin Nanoporous Silicon Membranes for Hemodialysis Applications Using Human Whole Blood Morteza Ahmadi Maud Gorbet John T.W. Yeow Department of Systems Design Engineering and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ont. , Canada Summary Two pnc-Si chips mounted in teflon block with blood flowing between the chips and dialysate flowing…
This is an update to my last post, where HUVECs mixed with collagen inside PDMS channels seemed to stay rounded up and not really do much of anything exciting. This time around I made a few adjustments: New collagen protocol – gives 1.5 mg/mL solution before mixing with cells: -10% 10x PBS -1.4% NaOH -38.6%…
In a previous post I had shown the relative fluorescence of various substrates as normalized to glass. 1um beads (either green or red) were placed onto each surface and imaged with constant EM Gain, exposure time, and intensity. Bar graphs were constructed to show the relative fluorescence (red and green were not normalized to each other)….
In this previous post (also some in this post), I had displayed the difference between the 120nm SiO2 microporous membranes and four different iterations of Track-Etched insert membranes in reference to nuclear (DAPI, blue) and actin (Phalloidin, green) stain. I recently performed the same staining protocol on other substrates: High Pore Density Microporous 300nm SiO2, Low…
I’ve redesigned the co-culture devices described in an earlier post to allow for media isolation as well as to alleviate concerns regarding bubble trapping. The new design is pictured below: The large cube of silgard builds a cylindrical reservoir for media and can support upwards of 100 uL of fluid (equivalent to other co-culture devices…
After last week’s post detailing the problems I’ve been having with my streaming potential setup, Jirachai was kind enough to come by the U of R last friday to look at my device. We banged our heads into the wall for a good two hours, but the drifting potentials and apparent insensitivity of the streaming…
A two multi-channel chip device was fabricated with gaskets and PDMS caps. The Upper fluidic