As I mentioned in the meeting this morning the SC267 samples have run out so I have begun testing SC269 for use in my experiments. This post pertains to a BSA diffusion experiment that I set up yesterday and allowed to run overnight. I took two SC269 samples; “treated” the first for 20min at 150C…

The following is a plot of rhodamine diffusion for the experimental system at various time point: In this figure, the rhodamine approaches equilibrium, as defined as a filtrate/retentate value of 1.  The 9x well chips have a larger active area and quicker diffusion. I have performed simulations of this system using COMSOL, and in this…

Hi all, this is Henry. Leeanna Hyacinth, an undergraduate student from the McNair program, has recently completed a joint project between the Waugh, Palis, and McGrath Lab to create a bioreactor to amplify the number of mature red blood cells. Background: The Palis lab has discovered a method to amplify erythroid precursor cells (from 1…

I am having some problems with my electroosmosis device and I was hoping that people with more knowledge about the TEM chips might have some suggestions.  For these trials, I used membranes from a wafer labeled 412-S.  I am still using the PDMS device from my last post although now I have mini “clamps” that…

Due to the discrepancies between the 1D model and experimental runs with rhodamine, I have switched over to using COMSOL to solve for a 3D model.  In this system, 200×200 um membrane squares with a 45 degree etch separate the two wells.   The top well is set up with .5 mol/m3 of rhodamine, and the bottom…

In the last month, we’ve run several sets of 15 nm pnc-Si wafers over a range of RTP temperatures.  Below is a chart that illustrates the current pore size control we have by tuning annealing temp. The 900 C, 950 C, and 1000 C samples were from wafers SC 231, SC 314, and SC 316,…

Building on my recent posts I ran an experiment with SC267 testing the ability of BSA and DDM to diffuse through UV-Ozone treated (20min at 150C) and untreated samples. I used four samples testing both BSA and DDM in the treated and untreated cases using Jess’ diffusion cell. Again 6uL was placed on the membranes…

In order to further test the model, I’ve set up additional rhodamine diffusion assays.  In each case, the setup is similar to the nanoparticle/protein/dna separations –  20 uL of .5 mM rhodamine on the top of a SepCon and 20 uL .1 M KCl on the bottom.  Two different membrane geometries were used so far…

In my last post I presented the results of an experiment in which it appeared that BSA did not diffuse through a SC267 sample treated in the UV-Ozone system for 20min at 150C. As a control I also ran BSA dissusion through an untreated SC267 sample and showed that BSA did move across the membrane….

Frustration with the lack of agreement with experimental device has led me to check if the diffusion in the wells within the model is physically relavent.  To do so I’ve solved a partial differential equation pertaining to a concentration of 1 on the left half of the system and a concentration of 0 on the…