Since HUVEC morphology hasn’t looked good in short-term adhesion assays recently, I analyzed HUVEC on the 2nd day after cell seeding. Normally, CMFDA staining is done for 30-45 minutes in serum-free media. I wondered if the serum-free environment during staining was affecting the HUVEC. So, I stained one sample with 5uM CMFDA in serum free…
After four days of this ongoing HUVEC culture in pncSi transwell, HUVECs look much better than before. Here I have freshly stained them with Calcein AM and we can see how bright it is in comparison to CMFDA stained cells (or may be there’s some flaw in my CMFDA staining procedure). Till now its been…
There’s been a lot of production in the last month and we’re still sorting through the data, but here is a synopsis of some key findings. All films were deposited with the AJA tool. Film reproducibility Week-to-week variation in pore morphology has never been noted explicitly due to the rapidly changing conditions we place on…
On Day 2 of cell growth from trial 3 of adhesion study, cells looked much more spread out but less in density. Most of the cells were observed to have concentrated near the edge of the pnc-Si chip near the Sepcon as seen in the figure below (first image in the bottom row). This could…
Samples (no RTP) from W673 and W684 were taken (four from each wafer; with atleast one blank chip) in Sepcons and HUVECs were plated @ ~19,000 cells/sq. cm. density. Cells were allowed to adhere for over 4 hours and this time stained with CMFDA (instead of Calcein). Cell types loaded with the CellTracker probes are…
According to recent cell adhesion studies on aminosilanized pncSi, no significant difference between adherence to the silanized and non-silanized surfaces was found. This could have been due to the adsorption of protein from the cell growth media onto the surface nullifying the effect due to silanization which is supposed to make it hydrophobic. ‘If silane…
This was my first experiment in which the Sepcons were inverted for cell adhesion. After allowing the cells to attach for 4.5 hours, I inverted the Sepcons in media for staining with CMFDA. Therefore, the Sepcon is set up so that the pnc-Si side is down and the cells are “upside down”. This makes the…
I repeated the previous experiment, this time plating slightly higher density of cells: ~15100 cells/sq.cm. Samples were from the same W673, no RTP) After counting the cells stained using Calcein AM, % Adhesion w.r.t the cells plated were found to be: non-silanized samples = 55.87%; silanized samples = 41.33% In above figures, not many cells…
This is something that’s been living on my knowledge page, but I think it’s a good idea to put it up here to get some discussion on it. Probstein gives the following equation for electroosmotic velocity in his book Physicochemical Hydrodynamics: U=-εζE/μ where U is the velocity, ε the permittivity, E the electric field, ζ…
I’ve been working over the holiday on trying to make Ag/AgCl electrodes. Here’s the method I’ve come up with so far: 1. 99.99% silver wire is used as the anode (+) and platinum as the cathod (-). 2. Silver wire is coated with chloride in a 1M HCl solution at a constant current of 10…