After recent studies with surface treatments, we wanted to take a step further by trying amino silanization on RTP’ed samples. Wafer 413 samples were RTP’d with no intact slits. Both 2D and 3D formats were tested in DMEM (-FBS) in oven @37C. All 3D samples had membrane side facing down whereas, in 2D format the…
This is some preliminary data that I put into my F32 postdoc fellowship application. It is a comparison of rhodamine transport through pnc-Si and PET transwells. These studies were done with PBS as the buffer. Sample volume = .1mL, donor volume = .3mL, receiver volume = 1mL, initial [rhodamine] = .5mM. I set the experiments…
A bunch of samples from W683 (no RTP) were liquid silanized and used in 3D format to track discoloration in comparison to control sample, in DMEM(-FBS) @ 37C in Oven. I used all the cracked chips which broke while clamping them into Sepcons. Instead of tossing them off, I just wanted to repeat the discoloration…
Surface treatments like silanization were seen to slow down discoloration in the past. I carried out liquid amino-silanization (protocol in place) of samples from W683 (no RTP) and used them in both 2D and 3D format to monitor discoloration in DMEM(-FBS) @ 37C in Oven. Also BSA coated samples were used side by side for…
There were some ideas being thrown around a couple weeks ago about discoloration results. I tested a couple of them out last week. These experiments were with w615 pieces, in the 37C oven in serum-free DMEM. I tested if vacuum grease or nailpolish would cause discoloration, I repeated the membrane up vs. down experiments that…
I did some imaging on the URinc TEM today and took the nice image below from wafer SC021. I wanted to see the correlation between highly diffracting (dark) crystals in our standard lower resolution TEM and the lattice fringes observed at high-res. In the image, the very strong <111> lattice fringe area to the upper…
Recently, I’ve been frustrated with polycarbonate transwells since they are opaque and I couldn’t see cells on them. So, I bought new transwells from BD (Falcon, PET membrane, 0.4um pores) and decided to test these out. I also wanted to see if coating the transwells with gelatin would promote cell adhesion. These are P6 HUVECs…
I carried out a repetition of BSA coating experiment with both 2D and 3D formats. With 2D, I get the same result as before, but surprisingly, in Sepcon 3D format we observe the opposite to what we see in 2D! (can be seen in the figure below) All the three samples with different BSA coatings…
I coated the pncSi samples from W683 (no RTP) with Bovine Serum Albumin (BSA) in Tris-EDTA buffer. Three different concentrations were used: 1 mg/ml, 5 mg/ml and 10 mg/ml (usual range of BSA conc. used for coating Si surfaces). The samples were left in BSA solution for 45-60 mins at room temperature and almost dried,…
Repeated the previous experiment with W683 and following images were captured after 5 hours of incubation in DMEM(-FBS), Oven @ 37C. I took Sepcon samples with regular thin o-rings, fat o-ring, without o-ring and w/grease in place of o-rings. Also for comparison, used cloning rings and the 2D flat format (left=w/o grease, right=w/grease) samples. All…