We know: W683 has no visible pores, W685 has usual porosity, and the recent Wafer 747 is ridden with pinholes. I wanted to test this new wafer for discoloration in comparison to the other two and got the expected result. In the figure below, there are two sets of images of same samples taken at…
The image below was taken with UR’s new S/TEM, the FEI Technai F20. It has the capability of resolving lattice images which should benefit the materials development effort. However, we will still rely on Karen’s TEM for most of our imaging since the throughput is higher on that tool.
Again a repeat, with comparison to the 2D format. To me, it looks like its again a repeat of what we saw in the previous trials. The sample with both intact slits has slightly higher discoloration than the others and also the pattern seems to originate from the slits (this is what we are looking…
Again a repeat of experiment and the observations. As seen above, the sample with both intact slits discolors the fastest (in Sepcon w/memb side down, DMEM w/o FBS, Oven @ 37C). Result is same as the previous two trials. One thing that I noticed is that the membrane in ‘one slit intact’ sample remains intact…
In the previous post, I used a compound error bar that took into account both image and operator variability. While that seemed to make things a little better, we were still having a problem with w705. I reprocessed a couple extra images from the TEM gallery, and added the differences to the image variability part…
I wanted to attempt pressurized and diffusion separations of brain extract using the new movable wafers. I tried pressure cell separations of 1:20 BBEC (clarified brain extract) at 2psi for 40 min. The flow rates were better than the standards I was previously using – this could be due to the little bit of glycerol…
I repeated a previous experiment but this time I used samples (no RTP) from W685 in oven @ 37C in DMEM(-FBS). Four type of samples (blank chip with no slits, chip with both intact membranes, chip with one intact membrane and chip with both broken membranes) were used at different time points. Again the expected…
We have known, W683: no visible pores; W684: very few pores; W685: usual porosity. After Vlassak’s view on discoloration, I took samples from above wafers to check if any difference in discoloration can be seen as they have different porosities. We could expect the more porous material to degrade faster than the less porous and…
Last week when I reported no membrane breakage after a week of HUVEC culture, I showed a phase micrograph on day 7. This week I tracked the HUVECs a bit more closely with the microscope. This is 10X, HUVEC P5, initial seeding density of 32,500 cells/insert. I tried to capture approximately the same area on…
Previous Post The previous w670 pressurized flow gels showed one trial with a lower cutoff than w673 and one trial with nothing passing through. This experiment was run for 40 min and in each case I saw only 0-3 uL fluid passing. I used the last couple of samples from this wafer to attempt a…