Previous Post We discussed in meeting having Maryna also calculating the permeabilities for this figure. Here is a figure with the experimental, theoretical permeabilities from Dave’s, Maryna’s, and my processing, and an average of the three theoretical permeabilities. I then took this average and made the following figure. I did omit w312, as the super…
After much anticipation, we have confirmed that pores do form with the AJA deposition system! Below are a series of images of membranes of two different thicknesses that were annealed at several temperatures in the RTP. 1000 C RTP; 40/9/40 stack Amorphous; 40/9/40 stack 1000 C; 40/15/40 stack 800 C; 40/15/40 stack Some immediate observations:…
My last cell post involved pictures of HUVECs after 1 day of growth on commercial transwells and pnc-Si Sepcons. Over the past week, I’ve been growing HUVEC on commercial PC transwells and pnc-Si in the Sepcon format in order to track the TEER. To get more data out of these cells, I decided to do…
I repeated the autoclave experiment from earlier this week, but this time I also used the Sepcon format. I used wafer 662, which was contaminated with large spots. This was done in the oven in RPMI-1640 with FBS (no RTP). I think my 0 hour pictures were accidentally erased from the camera, but I think…
I am not sure what prompted me to carry out this experiment. I took few samples from W703 (No RTP) and put them in petri dishes with DMEM (-FBS) at room temperature. I had two samples in a dish, with one flipped over (well side up). The sample with well side up discolored much earlier…
We have seen that discoloration of chips is slower in Sepcons and this has been speculated to be because of the separation between the ‘well side’ and the ‘membrane side’ of the chip existing in the Sepcon format. To further provide evidence to this theory, I carried out an experiment monitoring the discoloration of samples…
My previous discoloration post showed that chips in the Sepcon format discolored much slower than flat chips. A flat chip in the same well as a Sepcon chip seemed to accelerate discoloration. One thing about the Sepcon format that I hadn’t thought of earlier was that pnc-Si in Sepcons is autoclaved for sterility. So I…
Previous Post on Water Permeability Figure We decided at the last meeting to show water permeability for each membrane used rather than plot for a specific characteristic like porosity. I did this but there were a few samples that still had a noticeable deviation between experimental and calculated permeabilities. I asked Dave to reprocess the…
Cellulose Membranes I set up a 24 hour diffusion experiment with Millipore 30kD cellulose membranes as shown in this picture: After 24 hours I collected retentate and filtrate and visualized on the following gel: I didn’t expect to see a separation so similar to our pnc-Si results in this format considering the extremely poor dialysis…
I’ve been doing some preliminary studies with HUVEC in commercial transwells and on pnc-Si in the Sepcon/transwell hybrid format. The thinness of pnc-Si will enable better microscopic examination of cells than other membrane systems (if pnc-Si is flat). I thought it would be interesting to compare commercial transwells to the pnc-Si “hybrid transwell” format in 24-well plates via…