I ordered some new AC160 tips last week from Asylum and got on the AFM Thursday to test them out. I think they solved some of the problems I was running into over the last few weeks. Mainly, I was finally able to image entirely in repulsive mode (which was impossible with the older tips). All of…
Back a long time ago, I had performed some separations with the aim of showing the usefulness of pnc-Si in hemodialysis. I attempted separations of albumin (an important protein to be kept in the blood) and cytochrome c (used in hemodialysis research to cheaply approximate the toxic protein b2microglobulin) using three different membranes. Here is…
Hi all, the technique of UV Ozone bonding (of PDMS to glass, Si or pnc-Si) is now complete, serendipitously. Hsin from Miller lab want to bond PDMS to glass, they tried plasma oxidation treatment and it worked. The method is as followed: plasma oxidation treatment for 35 sec (which did not bond the sample, when…
Here is the figure we talked about in the last meeting. For each specific membrane, I’ve plotted both the experimental permeability and the permeability calculated from the theory. You can see in some cases they are close, but in others they can be pretty far off. Omitted from this chart are retests. Updated: Graph is…
Follow-up to Pressurized Movable Cutoffs. Wafers 670 and 673 were not holding up in PBS for the 24 hour diffusion experiment duration. RTP treatment at 800C for 60s enabled chips from both of these wafers to last 24 hours. Here are the results from the gels: We definitely have two different cutoffs in these experiments. …
I performed a discoloration experiment with w639 in EGM + 10% FBS. I put 2 blank chips in the Sepcons membrane side down. I then placed one Sepcon in a well with EGM alone. The other Sepcon was placed in a well with EGM but also with another chip (labelled as Sepcon with chip, chip…
The Bernhard group wants to use our membranes to separate pieces of DNA. During their experiments they noticed that some of their samples caused discoloration over the summer. Since then, I’ve been helping a new student to the project, Paul Black. He first started by testing pH, and found that higher pH leads to fast…
This is a repeat of the previous array experiment, but with a higher NaHCO3 concentration (1.85 mg/mL). Also, since these experiments require a lot of samples, I decided to cut chips off of wafer skeletons. This one was w622. As before, these were not RTP’ed and they were placed in the oven at 37C. I…
This past week I ran multiple experiments using the pressure cell and sep-cons. I used membranes from W684 for the sep-cons since the burst pressure of these memebranes is high (around 3.65-10Psi). I added 100uL BSA to the Sep-Con and put 20uL 1xPBS on the backside. Before adding the NC to the sep-con, I assembled…
Based on last week’s lab meeting, I made a “solution array” to test discoloration. Specifically, I made 3 solutions of NaHCO3 (0, .23125, .4625, .925 mg/mL) and 3 solutions of NaCl (0.1, 0.5, 1 M). I placed these non-RTP’ed w648 chips in the oven at 37C and took pictures at 4, 18, 72 and 120…