As per Jim’s suggestion, I coated wafers 335 and 341 with 40.6nm SiO2 on top and 42nm SiO2 at backside. The membrane cells have been passed to Anant and Maryna for chemical stability and air flow tests. Today I got some TEM images from the medical center with the help from Karen.We first imaged wafer341…

Just to double check the numbers I calculated on Tuesday I ran some wet-wet trials with 100K and 300K Nanoseps. Based on the wet-dry results we would expect that the permeability for the 100K Nanosep would be higher than that of the 100K Microcon. It goes without saying that the 300K Nanosep would also be…

UV Ozone treated W404 sample testing (benchtop) : In EGM In L15 In DMEM In each row, the first two samples (from left) are UV Ozone treated whereas the next two are not.It can be observed that there is no significant difference in discoloration due to the treatment after ~15 hrs. Note: The starting color…

W407 (non treated) in different NaCl salt concentrations (benchtop) : At t=0 In order (from l-r)1. 0.5 M NaCl2. 1.0 M NaCl3. 2.0 M NaCl At t=24 hrs. At t=53 hrs. At t=6 hrs and t=12 hrs the chips were not found to be discolored.This was carried out to test the stability of the membranes…

Membrane stabilityNon treatedW404 samples in DMEM 15um beads on the membrane:15um beads below the slit:In order: (left to right)1. t=02. t=33. t=64. t=105. t=13 hrs.Non treated W336 samples in DMEM15um beads on the membrane:15um beads below the slit:In order: (left to right)1. t=02. t=123. t=264. t=315. t=48 hrs.Chip discolorationNon treated W404 samples in DMEMIn order:…

A while ago I made a post about the permeability through the 100K microcons in the wet-wet EQ format. I had run three membranes, and stopped (and restarted) the centrifugation at three separate time points to be able to calculate three permeability values for each membrane. When I did this I got strange results; the…

I tested ozone treatment influence on the membrane strength and air permeability. The result: didn’t see any difference between ozone treated and non-treated samples. Burst pressure experiments description: tried wafers 391, 393, 344, and 345. Ozone treatment for 5 and 15 min didn’t show any difference. Air permeability: tested samples from wafers 344, 345 with…

Above video shows the disruption of the membrane from W404 after around 12 hours in the DMEM media (benchtop). The membrane was not treated with UV-Ozone and degraded pretty early as compared to that from W336 which broke at ~28 hrs. The membranes were loaded with 15um beads to check if they were intact and…

Maryna told me the UV/ozone cleaner did not work (the blue light in front of the chamber did not light up) when she was working with Dave today. I quickly checked the machine and it is actually working properly. The trick is to make sure you trigger the “safety lock” behind the chamber before you…