From SiMPore’s Basecamp thread … These are the nanopore characteristics. So between 14% and 20% porosity across the wafer and between 43 nm and 50 nm average pore size. So a little under-sized. I hope T-Cells and Monocytes can get through! 0.625% microporosity 0.625% microporosity
Introduction Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid with known paradoxical effects on endothelial cell (EC) barrier regulation. S1P binds 5 GPCRs: S1PR1 (Endothelial differentiation gene (EDG)-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8). Despite the aliases, endothelial cells predominantly express S1PR1&3. S1PR1 has been shown to have barrier protective or enhancing effects on…
We recently received 100 Dual Scale Membrane (lot 1383). As noted in Dan’s post we also had same issues regarding mix up in the labeling. In the gel box, 2 boxes (25 membranes/box) are labeled 0.625% porosity and 2boxes are labeled 1.25% porosity, thus we supposed to have 50 membranes for both porosity. However 1…
Recently we have received dual scale (nanoporous and microporous) membranes from SiMPore at two porosities: 0.625% and 1.25%. These feature 3 µm circular pores (Lot 1383). We routinely perform QC checks on membrane deliveries, so the following is report on this delivery. First, I’d like to note that we were supposed to receive equal amounts…
Introduction My previous post focused on attempts to reproducibly generate collagen IV with human umbilical vein endothelial cells (HUVECs) so that we would be able to establish protocols for basement membrane protein detection and degradation measurement. Following my presentation on November 18 2020, there were some questions regarding whether or not the observed collagen IV…
1. Introduction Fluid flow is an influential component of vascular models for conditioning cells and introducing other components such as leukocytes. In the previous post, we described our modular design for integrating a flow module into the uSiM device. Here, the simulated fluid flow and its experimental validation using particle image velocimetry (PIV) technique are…
Introduction This post will detail the work completed in the last chapter of my Thesis. The overarching goal of this chapter was to perform dialysis on whole blood utilizing a serial-separation system. This system would first: generate plasma from whole blood and second: remove uremic toxins from plasma employing microporous and nanoporous membranes, respectively. Conducting…
Since the arrival of ALine devices on the diagnostic scene during the SARS-CoV-2 sensor project, much of my effort has been focused on getting them to work properly with biological targets. With plenty of data showing successful operation of these devices with beads and several failures with SARs-CoV-2 surrogate particles, we have switched our focus…
The initial purpose of exploring formlabs SLA 3D printer for a collaboration with Dr. Nicola Marchi (link to previous post). The purpose of the this work was gaining the ability to use a simple membrane on 3D printed platform for BBB modeling and WBC migration though endothelial (and glial cell layers) and collecting and…
Hello All, I wished to be writing this from some far away tropical resort, as I was told PhD students of the past used to do before they defended their theses. Instead, this comes straight from the ROC homeland, deep in the midst of a global pandemic and a tea-fueled thesis writing session filled with…