Hi everyone, this is a short post quickly detailing some interesting quirks of different wafers as their respective chips are subjected to a control experiment (1x PBS, Qs = 30 ul/min, Qu= 10% Qs every 45 minutes). First, lets look at 1256 (2.7% porosity, avg. pore diameter 33.2 nm). These results are a nice baseline…

BMES – Atlanta 10/17/18-10/20/18 F-actin Alignment Quantification Using HSB Color Coded Maps ImageJ Plugin: OrientationJ Purpose: Quantification of F-actin alignment using ICC. HSB map provides a visual representation of fiber alignment with color codes related to hue:local orientation, saturation:coherency, brightness:fiber stain intensity (Figure 1). Our use: Quantification of HUVEC alignment before and after flow. Fluorescent…

With the installation of the new STED (STimulated Emission Depletion) system in the medical center, I have finally been able to work on imaging individual exosomes (that we know are exosomes because they are labeled for exosomal markers) that have been captured on our membranes. Now, I won’t go fully into the details of how…

Summary I need to replace the function of my breath to get water into the femtoliter sized cavity of the monolithic device. I achieve this using a small stream of ethanol vapor as a precursor, and then replace the ethanol with water. Once the chips are wet, they should be reasonably stable until they can…

Introduction In severe sepsis, a primary infection gives way to a maladaptive immune response leading to wide spread edema and multiple organ dysfunction. The initial infection can occur in many peripheral tissues throughout the body. The pathogen associated molecular patterns (e.g. LPS on gram-negative bacteria) interact with pattern recognizing receptors on resident macrophages and endothelial…

Alec and Elysia’s study on the migration of infectious bugs through microporous silicon nitride has been useful, however, the size range of the pores is not sufficient to halt the migration. To create smaller pore sizes, I infilled the chips from Wafer 4695 (600 nm pores) with SiO2 depositions. Deposition Settings 350 C Aluminum Holder…

Hello Everyone, This is an update on all my experiments and analysis on PKH paper. As i had a post before about PKH nanoparticles and PKH-Exosome fusion after labelling, we’ve been working on analysis and finishing figures for this paper. Figure 1;Observation of PKH nanoparticles by fluorescent imaging. A and B) Fluorescent images of PKH-labelled…

What happens to cells when they are exposed to a series of bubbles with and without a dubbler? The purpose of this experiment was to prove the hypothesis that bubbles damage cells, and the dubbler prevents cells from experiencing bubble damage. The experiment was performed with a PBS flow rate of about 0.14 mL/min through…

This post serves as a summary of the work done during my rotation in the McGrath lab this summer. Over the course of six weeks, I shadowed projects covered in the lab along with the specializations of microfluidic devices and porous membranes. I worked closely with Tejas, who gave me a glimpse of his work…

Hi everyone, in this update I’m going to go over some of the highlights from my experiments over the last month. After going through several trials that ultimately ended in failure, we moved over to testing long term performance of a 2.7% porosity nano-porous membrane (avg. pore diameter of 33.2 nm) by assessing it in…