I have had a chance to play with some of the chips from Lot 1278, specifically the design with the 2 micron slots. Results are a bit of a mixed bag. Wetting these chips continues to be a challenge. My first attempt to use the vacuum wetting protocol in ethanol resulted in a broken membrane,…
Introduction Since development, much μ-NPN work has been focused on observing cell adherence under flow, cell morphology on deferentially spaced pores, and neutrophil transmigration. This post will detail the entirety of my work with these membranes to date, and plans going forward. Previous Membranes Prior to μ-NPN development, we worked primarily with the microporous SiO2 and NPN….
The purpose of this device is to make uniform and straight scratch wounds in a 96 and 24 well plate for migration experiments. Previously, scratch wounds were made by hand using a p-10 pipette tip by scraping the tip in a straight line along the bottom of the well. Not only is manually scratching the…
For this post, Henry and I collected data for hemolysis and NTA sizing of soluble graphene oxide (GO). Hemolysis– prior work on the hemolysis testing of immobilized GO The purpose of the hemolysis experiment was to confirm that immobilized GO is the preferred from of GO for use in a portable hemodialysis machine. It…
For my work with Kyle and Vincent in Ottawa, I wanted to showcase more of the interesting configurations of DNA rather than simply passing the DNA through the nanomembrane. DNA Dual End Blockage and Clearage: DNA Flossing: DNA Stuck to nanofilter: These are all fine to show the pore being blocked at…
[latexpage] The relationship between the inlet and exit concentrations in a single pass over a membranes can be derived assuming first order loss to the membrane … \begin{equation} \frac{dC(x)}{dt} = -\kappa C(x) \end{equation} where is the local bulk concentration above the membrane and is the ‘elimination rate constant,’ a concept also used in drug delivery….
Hello Everyone, The goal of these experiments was to study the effect of exosomes on wound healing. We wanted to study, first importance of exosomes for migration of FBs and eventually closure (healing) of the wound and second effect of exosomes derived from ADSCs on FBs migration. In order to test our hypothesis, we designed…
What is it? A device to remove bubbles from microfluidic lines. Air and liquid enter on one side and only the liquid leaves. Our debubbler is disposable, cheap (<$0.50) and easy to make in the lab. Why use a debubbler? Bubbles can have a severe negative effect on cells cultured in a microfluidic chamber. The…
Hello Everyone, So Now that we have NanoSight in our lab, i decided to run some experiments with different conditions of media and compare the number of particles in each media. We designed this set of experiments like: Water DMEM DMEM + Exosome Free FBS DMEM + FBS ADSC Conditioned Media HDFB Conditioned Media HUVEC…
[latexpage] Hello Everyone, So i decided to do some calculations on our 2 different sets of experiments (Track Etched and NPN) and compare the applied and the required force of them, in addition to making sure that we are applying enough force on the particles to keep them captured. The experiment conditions for both TE…