We’ve calculated our instantaneous clearance numbers for the benchtop studies that use sepcon chips. Even calculating per unit area, our chips are not clearing much volume in these experiments. I believe this is due to the very low flow rate (1 µL/min). Kayli is starting experiments to use higher flow rates with the same chips…
Kilean and I are working on submitting a paper to Membranes on some of our electron tomography work. Here’s some of the work that we’ve done so far: Tomography Paper V2_KL
Introduction As a continuation to my previous post, I am running a triplicate of cell culture adhesion tests to observe cell lose on our membranes following physiological shear stress. Methods HUVECs (P3,4,5) were seed within our mimetic and left to grow to confluent for 24 hours. Shear (4.5 dyn/cm2) was initiated and images were collected…
We have a wafer that did not clear 500 nm pores (400 nm thick silicon nitride, #4682). There are about 230 chips that could be useful if they were etched a little. I used a SF6 based recipe (15 sccms O2, 30 sccms SF6, 10 sccms CHF3, 110/10 W FWD/REF power, 5e-5 torr base pressure,…
Microdialysis: Separation of BSA and Cyto C For the past few months I have been studying how well the 75 nm NPN-O membranes are at clearing cyto c while retaining BSA with and without the presence of PEG coating. Additionally, conductivity tests were performed to determine how well the membrane cleared a small molecule. See…
So, I have been working with a 4-probe TEER measurement system to record the changes in endothelial permeability during neutrophil extravasation and other biochemical assays such as thrombin treatment and TNF stimulation. 4-probe systems are sensitive because they don’t suffer from the contact impedances and measure only the potential drop across the sample under consideration. See…
As the first phase of my thesis, I have been working on parylene C membranes as potential easy-to-make thin polymeric membranes for blood brain barrier models following Dr. Carter’s works. The initial goal is to create membranes with different thicknesses with a reliable technique. Method First, the wafers are coated with micro-90 as a sacrificial…
[latexpage] This will be at least a three part series. Here in Part I, we will establish why adding flow to electrophoretic sensor (ssNP) is a bad idea. In Part II we will show why microfluidics is a benefit to adhesion-based sensors. In Part III we will explore the ability of a NPN/ssNP dual membrane…
Introduction/Motivation A problem many of us face in day to day research is the ability to change membrane format in a days to weeks notice. The cost of developing an entire new wafer of membranes for preliminary experiments is costly and time consuming. This cost and time increases when complex formatting is desired. All of…
Hi all, recently we improved cell tracking with the use of SiR-DNA label (see highlights below). random migration: A. Representative cell tracking from three independent migration experiments, color-coded by the color bars to the left in red, green, and blue. B. Distributions of motility parameters (persistence time, instantaneous speed, average speed, pathlength, and displacement)…