In the past, I have been working on developing mechanisms to inhibit neutrophil migration through endothelial basement membrane. Please find the previous blogs on the following links https://trace-bmps.org/updates-on-neutrophil-collagen-migration-experiments/ https://trace-bmps.org/manipulating-neutrophil-migration-through-the-vascular-barriers/ https://trace-bmps.org/sem-imaging-of-neutrophil-transmigration-through-collagen-gel/   This is a small update on the following. CD29 is beta-1 integrins, which are used by neutrophils and endothelial cells to adhere to basement…

Figure 2 – Micro scale Experiments with 10 micron fluorescent particles and 8 micron pore size track etched membranes: A) Fluorescent intensity – time plot showing increasing in the intensity signal during the capturing step and decreasing for releasing step. B,C and D) Before capturing, After capturing and After releasing panels, respectively, including schematic images…

So in the past couple of months, I have focused on wrapping up the microdialysis project that was initiated by Alec. Just as a recap, this experiment utilizes a modified SepCon that features an open bottom to allow for separation of albumin and cytochrome c via diffusion. 50 uL of 1 mg/mL BSA and cytochrome…

Motivation and Procedure After discovering that pores on 3um LP SiO2 membranes seem to get blocked following 3 hours of shear [of HUVECs], Kilean and I  decided to utilized SEM to further inspect what might be doing the occluding. HUVECs were seeded onto the membranes as usual and left to grow to confluent for 24…

Purpose: We want to know the effects of the RTP process on pore size, pore density for the biased films coming out of the AJA. I believe that the RTP ramp rate is important for forming bigger pores. Some work (Joe Qi Thesis) has indicated that the pores come out more circular as the wafer is…

Hi all, my name is John Cognetti, and I am a first-year PhD student in BME. I recently finished a rotation in the McGrath Lab. My goal was to measure changes in resistance across the membrane as the membrane becomes saturated with particles. Initially, the plan was to use Tejas’ trans-endothelial electrical resistance (TEER) setup…

[latexpage] This an extremely important topic that has come up multiple times in discussions within NRG as well as during my qualifying exam. The question is: are gold nanoparticles a good model for exosomes? The reason that this question comes up is because exosomes are biological material and as such they have some vastly different…

Traditional Wound Healing Analysis (the implicit gold standard) Wound recovery is reported base on the closing of the two wound edges.   Proposed Wound Healing Analysis (cell counting/tracking methods): Wound recovery is reported base on the migration/movement of the wound healing cells.   Recap on angle autocorrelation We look at the correlation of angles between…

In 5.4 mm single slot chips, sheep blood was flowed at 3 µL/min though the 3 mm x 1 mm channels with PBS flowed at the same rate but in the opposite direction for four hours. The chips were pre-wetted with PBS and rinsed following flow. The membrane surfaces were fixed with 4% paraformaldehyde for…

Hello Everyone, For the next figure in our paper, and to make the transition happens smoothly from Track Etched membranes to NPN membranes, we decided to run the same beads experiment on NPN membranes as well. So Kilean and i started working on these experiments, and the first experiment was to capture and release 100…