Hi All, These are documents associated with my proposal: Silicon Nanomembranes for Rapid Liquid Biopsies from Whole Blood and Urine. Proposal: Kilean_Lucas_PhD_Thesis_Proposal_Reduced File Presentation: Proposal Presentation_03

Hello Everyone, I have been trying to take SEM images in 3 different steps during my experiments with polystyrene beads over Track Etched membranes including before capturing, After capturing and releasing. Micro Scale Experiments:  As you might remember, in Micro-scale experiments, we use 10 micron beads and 8 micron pores. Before capturing: This is only…

Introduction Throughout my rotation, I have been working on the dialysis project with Alec. The goal was to set up a multi-pass system to analyze the membrane’s ability to separate different molecules in blood. The critical separation is between β2-microglobulin (12 kDa) and albumin (66 kDa) – β2-microglobulin is a large toxin that needs to…

Hello Everyone, Please note that Figures are all shown in the powerpoint file. The goal of these experiments was to plot a standard curve for concentration and intensity in my Track Etched Membranes. In order to that, we decided to dry droplets on the membranes with different dilution, then check the fluorescent intensity of them…

Intro Recently we have been collaborating with the Moghaddam lab from the University of Florida, which specializes in the fabrication of graphene oxide (GO) membrane, to study the use GO membrane as a toxin filter or absorbent in hemodialysis and live support devices. We are  responsible for the hemocompatibility component of the study and used the following…

With my TEM tilt stack work, we can see a marked difference in the roughness of npMg2 and NPN. Quantifying the bulk roughness properties of the npMgF2 using AFM gives us a way to check that property. Surface roughness has been known to effect SERS activity in other substrates, and our current hypothesis is that…

Introduction The on going work for the dialysis project at UR has undergone changes and many hours of trouble shooting over the past semester. We are at a point now that I would like to share all the work I have done up until this point including assay development, method progression, and the experiments we…

We’d like to get verification of the absorption spectra of our npAu/npMgF2 SERS structure. The SERS effect should occur where the absorbance is maximized. Total Light = Absorbance + Reflection||Scattering + Transmittance Last Friday, I had the opportunity to work with Jennifer Kruschwitz who possesses a Konica-Minolta integrating sphere spectrophotometer from 380-740 nm (10 nm…

The purpose of this study will be to compare the cellular adhesion on PEG vs Bare nano porous silicon nitride membranes using the same fluidic devices as the in vitro clearance study. The devices will need to be disassembled for imaging. Materials: 6 chips, 3 PEGylated, 3 Bare. gaskets for 6 devices Tygon tubing 2…

I recently attended ASN’s Kidney Week 2017 in New Orleans. I mostly ate beans and rice, although there are three completely vegan restaurants and several restaurants with vegan options. The music was terrific and the weather was terrific, the people were terrific, etc. I had Five objectives for my trip to this conference. 1. Meet…