The plot below shows a calibration curve to calculate the etching time needed during the RIE step (used to reduce the size of the nanospheres) to obtain a desired pore size. The membranes were patterned from nanospheres with a nominal diameter of 300 nm., we know from SEM images that their actual size is 340…

As the title states, I have been making TEM images and reconstructions from a variety of NPN materials. I hope to correlate the underlying nanostructure with SERS activity eventually. These images show many different porosities, topographies and pore shapes. Wafer 1236 has been the bulk majority of my npMgF2 fabrication for my SERS experiments.  …

So Alec, Tejas and I will have a poster at the Immune Imaging Symposium in the Med Center on Nov. 4th and we created an image for our abstract that Jim requested I post here in higher quality. Immune Imaging Abstract Image_01 The image is so high quality that it was having trouble loading into…

Last week, I attended the American Society of Exosomes and Microvesicles (ASEMV) conference in Monterey, Ca and presented a poster on the work that I’ve been doing. Despite a very low turnout for my poster session (due to the scheduling of a long break before and dinner after), I met with some very interesting people…

Hi all, This is a “take two” of the mock figure for our manuscript titled “Porous substrates influence early endothelial migration and the associated fibronectin fibrillogenesis”. Many new figures are produced based on the reviewer suggested experiments. It is likely that we will have the word”early” from our manuscript title. PS: The old, raw mock…

For last many months, I am working on neutrophil migration experiments to develop protocols to inhibit them from traversing the basement membrane. My previous blogs are here and here. I want to prevent neutrophils from crossing the endothelial basement membrane (BM), and record the changes in TEER and try to correlate with the gain in…

My goal and focus of the last post is to to use the existing SU-8 grid structure used on the ultrathin silicon oxide lift off membranes to create . To accomplish the goal the SU-8 surface needs to be modied to decrease cell adhesion so that the cells are trapped in the wells. Please see…

This post offers an update on membrane fabrication through nanosphere lithography (NSL), describing in more detail the releasing step as well as the membrane integration into PDMS devices. Membrane lift-off is achieved by dissolving a ZnO film underneath the membrane. After fabrication the sample is immersed in 1M HCl solution. The figure below shows a…

So this is basically an update of my post from a couple of weeks ago (and a figure from the poster that I just put up), but it is a very good status update of the contamination experiments that I have been running with XPS. Basically, I ran the sample that had been purified by…