Kilean re-imaged some of my old samples with neutrophil transmigrating through the collagen gel. This sample was prepared and imaged on June 25th, 2017, and kept under dry conditions. Surprisingly, the top gel kinda got delaminated and we can see the gel peeling off the top surface. The neutrophils can be seen on and below…
Background For the in vitro portion of our dialysis project here at UR, we have settled on using two major experiments to test our devices for eventual transition to an in vivo model. These experiments include a single pass system using multiple syringe pumps to observe the effects of fouling on our membrane as well…
The plan was to fabricate ~7nm pores on the PDMS-painted 200nm SiO2 + 20nm SiN chips and take IVs overtime, and observe the stability of the nanopore’s size. I fabricated a total of 8 200nm SiO2+20nm SiN chips using the Monolith protocol, to 7 nm. Half of the chips were wetted with the freezer step…
[NOTES] BMES 2017 highhlights
Here is a summary of some of the talks I attended at BMES 2017, mainly from the Nano and Micro- technologies track. This is an ongoing post, I will post more comments, links, and figures. Anna Kalmykov (Tzahi Cohen-Karni) Carnegie Mellon University: 3D Nanosensors for Electrical Interrogations of Engineered Micro-tissues. Overall synthesis of nanostructures to be used…
Hello Everyone, So following up on my exosome scale experiment and my last post, i am presenting my last update to summarize my results and to show that my results are trustable and reproducible. This is how we take images: We have different positions in the interaction area, and we take images as the experiment…
Some of the animation work that I have done (example as above) using Blender has been useful to communicate ideas and generate high quality images for papers. However, it is often time consuming to run them on our own personal laptops. The figures I generated for Kyle’s nanoporous tenting paper could take a day…
Hello Everyone, Kilean helped us to set a training session for NanoParticle Tracking Analysis (NTA) in UR Medical Center. For this training session, i had 4 samples including: Beads, Liposomes, Media + 10% serum, and PBS. Here is a summary of what we learned, i am also summarizing a paper that i found for comparing…
As the title states, these are images from the monolithically fabricated devices used by Kyle and Vincent in Ottawa. These devices were fractured with tweezers, then tilted and metallized with 5 nm of Pt, mounted on carbon tape.
I’ve now made 18 pores in monolith devices, with only 1 real success, which was the first one that I report on already. All of the rest have failed for various reasons, mainly relating to the silicon nitride membrane in which the sensing pore is fabricated. The first issue is fabrication time. Fabricating a pore…