This will be a very brief post, but kind of an interesting one. I have been attempting to get my identification of exosomes by immunogold labeling and that hasn’t been going terribly well. I think that it is due to a wealth of complications including a lack of fixed exosomes, salt contamination and other problems….
Hey all, I have the first draft of my ASEMV poster here for any feedback that you might have. The conference is in Monterey, CA next week and any feedback that you have on this by Tuesday or Wednesday evening of this week on the poster would be awesome. ASEMV_Poster_KL_09132017_01
What is SiR-DNA? It is a far-red DNA stain, known as SiR-Hoechst, and can be used for live-cell imaging. Most current DNA dyes are toxic or affect the cell’s motion or proliferation. https://www.nature.com/articles/ncomms9497 It does not affect the migration of human dermal fibroblast cells (hdFb). The motility parameters (speed and displacement) for a 24 hour…
Hello Everyone, I have been working with Track Etched membranes since last year and we always had problems with leaking and also not being able to push the flow through the membrane. At the end of the summer, i decided to test my new idea for my devices and finally i have a system which…
One of the major goals of my work with capturing exosomes is to show that our purified population is less contaminated with protein than exosomes purified by ultracentrifugation. This is important because the protein (and all the other junk floating in plasma such as cfDNA) can cause problems with identification and characterization of the exosomes….
Some of my recent cell culture work on Microporous MgF2 windows have shown vacancies in the cultured monolayer after fixation and staining. We should see a contiguous layer. To better understand my fixation, I seeded and cultured P10 RPE-19 cells at a high density on a coverglass and in a polystyrene dish for a week….
Introduction Endothelial cell detachment under flow on porous substrates has been a concern in our sepsis research since I began my work in the lab, and some time before that as well. As we plan on moving to microfluidic models that incorporate larger pore substrates and physiological shear stresses for extended periods of time, it…
As we run our CCD (Central Composite Design for Optimizing SERS Substrates, SERS Measurements on 150C, 250C, 350C 1236 npMgF2), we have also been gathering TEM/SEM structural information about pore distributions and MgF2 grain structure. This post contains images about materials and compares them to information we know about wafer 1085, one of our original MgF2…
There is no excerpt because this is a protected post.
This post includes some optical and SEM micrographs of a SiO2 membrane patterned by nansosphere lithography using 300 nm polystyrene beads. An SU-8 microgrid was deposited on top and the membrane was released by dissolving a bottom layer of ZnO. After the release, the membrane is rinsed in water and transferred to a clean substrate….