Carrie and I have been trying to detect HMM (APOBEC3G) diffusion through Sepcons without much luck. We determined that the dilution factor (~1:30) for diffusion in our 384-well plate format was too high to detect HMM in the basolateral well. To try to get around this problem, I decreased the volume (and thus dilution factor)…
Last week, I worked with Kevin to create a prototype cell culture device that could be compatible with his Raman system in Nottingham. The space constraints of the physical system are challenging: The overall working distance of the system must be less than 1.5 mm (smaller is better), must maintain only MgF2, media, and cells…
Author: Kevin Ling Dysfunction of the outer retinal blood barrier (oRBB), which is composed of the retinal pigment epithelium (RPE), Bruch’s membrane, and choriocapillaris (CC), is implicated in age-related macular degeneration (AMD), which causes irreversible central vision loss [1-4]. AMD is the leading cause of blindness in industrialized countries; it is estimated that by 2040,…
Alright, so this is kind of a big update and I will probably split it into a couple of parts. Basically, in a search for a way of confirming that the particles we are seeing on the membranes, I have tried several techniques with varying degrees of success. The initial experiment involved me conjugating an…
It took me a little while to figure out how to turn the .avi file into a .gif, but here is an image of the diffusion through 501. Due to a problem with MatLab, it took about 5min before I started taking images of the diffusion. So this starts at ~5 min after the 100mM…
Kim(Haynes)AnalChem12.pdf