war

Having finished the hydraulic permeability testing with DI water I wanted to see what the effect of an introduction of protein would have on the permeability values. To do this I simply reran a set of fresh membranes at 21.63psi using a 1mg/mL solution of bovine albumin in PBS instead of water.The group on the…

Separations were performed at 2 different salt concentrations with equal volume filtrate and retentate (to avoid dilution problem). Separation time was 40hrs rather than the usual 24. The lower salt concentration run has a lower cutoff – which is just like what we see in the DNA separations. Remember though that proteins have different charges…

Mary Lee and I performed a quick test to determine the efficacy of different surfactants at reducing agglomeration between IgG and 20 nm fluospheres. Each solution is a combination of 50 uL 10 mg/mL IgG (in PBS) + 50 uL 20 nm red Invitrogen fluospheres + 500 uL of surfactant. We used 1 x PBS…

As many of you know, we have been having a lot of trouble with the new etch cell and have not been able to produce high quality membranes. Yesterday, we discovered that the stainless steel used to fabricate the new cell is different than that of the original cell. Here are the EDAX spectra: Old…

Above video shows the disruption of the membrane from W404 after around 12 hours in the DMEM media (benchtop). The membrane was not treated with UV-Ozone and degraded pretty early as compared to that from W336 which broke at ~28 hrs. The membranes were loaded with 15um beads to check if they were intact and…