EO at 1 mM vs 100 mM salts & the role of a meniscus in pressure measurements

Two weeks ago (as of the time of writing this post), I wrote a post on a dead end filtration experiment that I ran in order to demonstrate particle capture in pores, modeling a simple, purified exosome capture system that we could use for imaging or analysis. However, when I ran that experiment, I done…

Goal: study HUVEC migration on different porous substrates. HP = high porosity 0.5 μm SiO2 membrane LP = low porosity 0.5 μm SiO2 membrane NP = non-porous SiO2 membrane TCP = tissue culture polystyrene (~3 GPa [ref 1, 2]) P184 = sylgard 184 (a commonly used PDMS; Young’s modulus = 1.74-1.84 MPa [ref 3]) P527 = sylgard 527…

In the field of gas permeance there is a plot called the “Robeson Plot” (the paper that debuted it) that is used to compare the separation behavior of various membranes made of different materials and measured in different systems. An example is pictured: Various literature data for the log permeability of H2 gas versus the…

This is a side view schematic of the experiments I’m setting up in Mike and Derek’s stir cell. The membrane separates two wells. In the well where the membrane is flush with the wafer I add 300uL 5nm gold in H2O. On the other side I add my buffer and a stir bar. Adding a…