w705 Permeability and Separation
W705 performed well in terms of permeability and separation of Hybrid Silica Nanoparticles. No water passed in the wet/dry format (no matter ozone or testing configuration – centrifugal or pressure cell). Reasonably high flow rates when tested in wet/wet configuration in the centrifuge. PVP enabled flow in the pressure cell in an otherwise wet/dry configuration. Permeability measurements are likely low for PVP because flow initially started very slowly and increased as the bottomside of the membrane became fully wetted.
Permeability in uL/cm2-min-psi
wet/dry centrifugal 0 (n=3)
wet/dry pressure cell 0 (n=2)
wet/dry (ozone) centrifugal 0 (n=3)
wet/wet centrifugal 1623 (n=3)
wet/wet (ozone) centrifugal 1231 (n=3)
wet/dry PVP pressure cell 278 (n=2)
wet/dry (ozone) PVP pressure cell 342 (n=2)
Particle Separations
The max pore size is reported to be approx 45 nm. So I tested for passage of 10 and 45 nm Hybrid Silica particles (which are not monodisperse, but have a small distribution as well).
Absorbance (@ 650 and 550 nm) – after about 100 uL of 500 uL fluid passage in a PVP setup
45 nm TRITC
Retentate 0.010 Filtrate 0.001
10 nm Cy5
Retentate 0.011 Filtrate 0.012
