Just wanted to show the detail of PETL channels. A couple of interesting things: The adhesive layer(s) are pretty thick, and look pretty smooth after heat curation. Also, the channels are slightly beveled, probably due to craft cutter blade.
In order from left to right: 1) t=0, huvec on pncSi, in EGM (10% FBS) 2) t=24 hrs (cells reach confluence) 3) DIC after staining 4) FITC (live cells) 5) Rhodamine (dead cells) Its good to see the cells reach confluence and the membranes intact at the same time! There is a decrease in number…
Introduction High throughput imaging techniques in conjunction with in vitro micro-physiological systems (MPS) allow for comprehensive exploration of physiologically relevant phenomena in a living 3D mimetic of a tissue microenvironment. Prior work in this lab has readily demonstrated that vascular tissue mimetics (the µSiM) facilitate real time phase-microscopy visualization of immune cell trafficking in response…
Here’s the paper we’re discussing this week: Wu(Moulin)JMS14 The authors motivate their work by claiming that ultrafiltration membranes can be used to keep “engineered nanomaterials” (ENM) out of our waters. They use confocal laser scanning microscopy to locate the position of fluorescent CdTe quantum dots (QD) on/inside an Ultracel membrane. (Made by Millipore using regenerated…
Wound healing experiments I have been isolating extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) and using them in wound healing experiments. For isolation, I follow the same protocol as outlined here. For these experiments, I used small EVs, which are collected in the last step of the isolation process. I began by seeding human…
Introduction As we’ve been trying for nanoparticle-based CAD-IA for multiple trials, we have tested various reaction routes, reaction conditions, and antibody pairs with 3 different nanoparticles. Here, I would like to take a step back to go through the CAD-IA stories based on different nanoparticles. CAD-IA based on different nanoparticles Fluorescent SA-modified magnetic particles Physical…
This week I tried to run a few “eq-tests” with some samples from wafer 187 to get some hydraulic permeability value for our membranes to see how they compare to the polymer filters used in the microcon and nanosep systems. Unfortunately I have no results to report, of the two samples I tested one broke…