To keep the NRG up-to-date on the goings-on in our collaboration with the University of Ottawa, we will plan to share our progress slide decks from the CRePE-ssNP group meetings that happen every two months. Periodically, we’ll also update the NRG on overall progress of the project including the work done in the two Ottawa labs and at SiMPore. I’ll present my slides via Keynote today, but here they are as a PDF.
We have an STTR grant funding us to revise the Sepcon plastic housing to enable us to do reverse centrifugation easier. As outlined in this previous post, we’re trying to remove unbound IgG from a solution of IgG-antibody conjugates using reverse centrifugation: The earlier work used Josh Winan’s jury-rigged PDMS-filled reverse centrifugation setup, but despite the…
To analyze the dimpled Sylgard substrates that we’ve been making, we’ve been conducting experiments to investigate how topographical differences in the surface of a substrate impacts HUVECs and ADSCs, in this case the dimples. We’ve already shown that cells will grow on these membranes in our “Dimpled Sylgard Membrane Device” post and that our substrates are wetting in our “Degassing Experiments”…
I’ve been working on trying to fill in some of the blanks of the permeability paper, and I’ve been fiddling with the water permeability data. I plotted the old water permeability data as a scatterplot, and while there looked like they could be a linear relationship with porosity, it was definitely missing some medium porosity…
The mechanism of organic dye photobleaching is not well understood, but there are a few methods out there for reducing the rate of photobleaching. Some literature suggests that photobleaching is due to excess oxygen in solution and the interaction between the dye and oxygen radicals. Actin research swears by the following “anti-bleaching buffer” to limit…
Wafer 416-T is a TEOS wafer annealed at 900 C. Pinhole density is relatively low. left: (1,0) @ 35kx; right (0,4) @ 35kx left: (1,0) @ 50kx; right (0,4) @ 50kx
I’ve made some modifications to my V2 design such that it allows total vertical access, among other things: This design utilizes embedded electrodes and filter within gasket layers to create a tight fluidic seal for each channel. Here, the slot is pointed upward, so the endothelials will be on the bottom side and the…