Counterflow Dialysis

I’ve been wondering if there were differences in actin/cytoskeletal structure of cells on supported vs. free-standing pnc-Si.  A couple weeks ago, I had a couple of extra pnc-Si transwells so I stained for actin. I followed the actin staining (rhodamine-phalloidin) method I uploaded to the protocols section of the lab, and I counterstained the nuclei…

Introduction Motivation: We want to assess if the bladder cancer cell line T24 would influence the baseline characteristics of bladder epithelial cells (BdEC, a.k.a. ATCC® PCS-420-010™) when the two cell types are cultured together. Ultimately, we are interested if the T24 induces BdEC malignancy (turning the normal epithelial cells cancerous). Goal: Investigate if BdEC monolayer will break…

Aminosilane has been used widely to derivatize the surface of silica for the applications in analytical chemistry, biochemistry, and biomedicine. It also plays an important role in providing adhesion for Nafion coating on silica surface. The basic principle is described as follows. After oxidizing the surface of silicon, the next step involves the derivatization of…

To identify how much of an effect the membrane has on particular simulations, we originally came up with the idea of using a phenomenological effective diffusion to view the membrane impact (see this post).  Here is the most up to date version of the figure, which was slotted for use in the simulated diffusion paper:…

Wet Etching of Optical Thin Films   As a result of Christian’s trip and Kevin’s discussions with me, I have been wondering if we could make nanoporous MgF2 membranes for Raman spectroscopy. Part of the appeal in using our Si-based nanomembranes is that the volume they occupy in the observation space is extremely minimal. Even…