Counterflow Dialysis

Hello Everyone, The goal of these experiments was to study the effect of exosomes on wound healing. We wanted to study, first importance of exosomes  for migration of FBs and eventually closure (healing) of the wound and second effect of exosomes derived from ADSCs on FBs migration. In order to test our hypothesis, we designed…

Microtechnologies for cells Phototunable Spatiotemporal Presentation of Viscoelastic and Adhesive Cues in HA Hydrogels Erica Hui1, Kathryn I. Gimeno2, Thomas H. Barker2, Steven R. Caliari1,2 Department of Chemical Engineering, Department of Biomedical Engineering, University of Virginia https://www.biorxiv.org/content/10.1101/646778v1.full Developed a hyaluronic acid (HA) hydrogel system to study the role that spatially-patterned matrix mechanical (viscoelastic) and adhesive…

Background Focal adhesions occur in 2D cell cultures as substrates apply large forces on cells. Previous experiments showed that focal adhesions formed on TCP and on non-porous SiO2 membranes but that less formed on 3.0µm-pore SiO2 porous membranes and even less on 0.5µm-pore SiO2 porous membranes. In other words, as area between pores decreased, formation…

Hey folks, Science Saturday is this Saturday and Sunday at the Rochester Science Center. We need volunteers to man our tables from 12-4pm on both Saturday and Sunday. Please indicate in the comments of this post if you can volunteer, and when you can volunteer. The tentative schedule is as follows: 12-1 Saturday – Greg,…

Bob and I are trying to determine which wet etch would be appropriate to use to remove the native oxide inside the pores before membrane liftoff. The issue is when removing the native oxide, we will remove part of the membrane. So far, we removed native oxide from bare silicon wafers using 50:1 HF. For…

I tested those membranes from w335 and w341 with approximately 40 nm of deposited oxide on both sides for air permeability and burst pressures. There was tiny amount of air flow (I checked for leak) from few of them which I didn’t expect with such thick oxide. The burst pressures are higher then without the…