Counterflow Dialysis

The following diagram lays out each separate setup that was tested using different combinations of Geltrex and substrates. Each trial has a different reasoning behind why it was done. The purpose of these trials was to investigate the effects of Gelrex gel and PCTE membrane inserts on tube formation in Bend.3 cells. First, we showed…

The goal of the design is to design a system that can co-culture cells under varying flow conditions, measure TEER, and perform dynamic microscopy. Specifically, the endothelial tight junctions are the primary mechanism by which the BBB is regulated. It is thought that astrocytes and pericytes can influence this mechanism. With the completed system, we…

Because of the disagreement between the experimental and simulation comparison in this post, I decided to take a look at the Sterlitech membranes using SEM.  Barrett gave me a hand with all the images, and we tried to look at the edge for thickness and the pores for density/overlapping pores. In this first image, the…

Hi all, Sometimes for flow modeling I assume all pores to be identical in size. However, since larger pores allow much higher passage of flow, the above assumption will generally underestimate the prediction of flow.   Presented here is a simple example, base on the following pore distribution (obtained from a SEM image): Assuming all…

Having finished the hydraulic permeability testing with DI water I wanted to see what the effect of an introduction of protein would have on the permeability values. To do this I simply reran a set of fresh membranes at 21.63psi using a 1mg/mL solution of bovine albumin in PBS instead of water.The group on the…