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Adipose Stem Cells on Nitride LiftOff Membranes (Part 2)
Background: In a prior post I showed that we had seeded cells on both nano- and micro-porous SiN Liftoff Membranes. The nanoporous proved to be rather weak and showed numerous broken windows after seeding the cells. Summary/Conclusions: In this follow up post, I seeded adipose derived stem cells on 50 nm thick microporous SiN liftoff membranes with…
Cell-substrate interaction modeling
The idea of this project started when we were writing a mini-review about cell-substrate interaction. Although most of studies of cellular responses to porous membranes have pointed towards a multiphasic response with the same trend of changes with respect to the pore sizes, we also found some variation in the results. We suggested that some…
Instantaneous Clearance per unit area – Animal Study
We’ve calculated our instantaneous clearance numbers for the benchtop studies that use sepcon chips. Even calculating per unit area, our chips are not clearing much volume in these experiments. I believe this is due to the very low flow rate (1 µL/min). Kayli is starting experiments to use higher flow rates with the same chips…
Permeability causes vacuole formation (2)
In my last post on this subject, I showed that cutting off permeability of pnc-Si chips inhibits vacuole formation in endothelial cells. To do this, I seeded cells on the membrane side, allowed them to attach, inverted them onto a media bubble contained within a cloning ring, wet the well-side with media and then placed…
Kidney Week!
I went to Kidney Week in Philly, Nov 13-15. American Society of Nephrology. I spent most of my time in the Bioengineering talks and posters. Here are some highlights. I put the Abstracts pdf on the cloud. Recellularization of Vascular and Tubular Compartments of Rat Kidney Scaffolds with Embryonic Stem Cells Basically, they perfused…
Alternate Smoluchowski Equation
My previous post on Electroomosis theory describes the deviation between expected and calculated zeta potential using the Smoluchowski equation. In this post I will explain another method of solving for the zeta potential and I will consider the mistakes made in the previous calculations. In a paper by Kim(Mukhtar) in J. Membrane Sci. (1996), the…
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Can we export ppt files to html and upload those files with a link? I prefer not to have download anything to read anything on the blog.
but my animations won’t show! ok, i changed it to a .pdf