Hi All,
Presented in this post is the following paper, titled: “Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices”
[REVIEW] exosome handling in micro and nano devices
Keynote presentation: [PRSENTATION] NRG (2015-11-16) exosomes
The coating was done last week at RIT (it was postponed from two weeks ago because of the failure of the e-beam crystal sensor). I deposited 10nm Ti and 15nm Au, without vacuum breaking. All the samples are from wafer #313. TEM images were taken yesterday with the help of Karen. (Her machine is really…
Surface treatments like silanization were seen to slow down discoloration in the past. I carried out liquid amino-silanization (protocol in place) of samples from W683 (no RTP) and used them in both 2D and 3D format to monitor discoloration in DMEM(-FBS) @ 37C in Oven. Also BSA coated samples were used side by side for…
Today I brought membranes and the streaming potential device to David Borkholder’s lab at RIT. His students and I set up an initial low voltage experiment. They will continue to test the device for flow rates and back pressures. Here is a list of methods and results from today: SC 214, RTPed but not oxidized,…
Introduction This experiment is a continuation of previous work, cited below. Instead of urea spiked 1x PBS, whole heparinized bovine blood was pushed through the device over a period of four hours to then perform a urea assay on the separated and collected plasma. Please refer to the post below regarding the experimental setup that…
A study of membrane deflection has been conducted using the Veeco Wyco optical profiler. This is the first time the Veeco has been used to obtain quantitative data describing membrane deflection. This type of analysis will be useful for several reasons: –It will provide a numerical measure of maximum deflection and deflection vs. position for…
Previous posts: Theory Deposition Etching Imaging I have tinkered with the etch recipe some more, adding Argon to help prevent polymer formation (adding 5% O2 was not enough). The current recipe is 200 mTorr (100 mTorr Ar, 5 mTorr O2, 95 mTorr CHF3) at 120-125 W (6-12 W reflected). The plasma color is initially rosy (Argon), settling into a…