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R61/R33 Update to NIH on Nov. 9, 2022

ByJim November 15, 2022

Attached is a PDF presented to the NIH at our annual update meeting that included other funded project teams.

R61_R33HL154249 11-22 Update (Low Res)

memBrains

10 kDa Small Molecule Permeability Optimization
ByMolly McCloskey November 15, 2020February 9, 2021
Introduction A healthy blood-brain barrier is not permissive to most small molecules, but during inflammation, tight junctions weaken and the barrier becomes more permissive. We have developed an in situ permeability assay that tracks diffusion across a cell monolayer and membrane and into the bottom channel of the µSiM device. The assay tracks a fluorescent…
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IMR90-4 EECM-BMEC-like Cell Analysis in µSiMs
ByMolly McCloskey October 21, 2021October 21, 2021
Introduction We are differentiating and validating EECM-BMEC (Extended Endothelial Cell Culture Method-Brain Microvascular Endothelial Cell)-like cells in µSiM devices. See this post and this post for method details and validation in traditional cell culturing platforms. Methods Attempt 1 – Overstimulated cells IMR90-4 iPSC EECM-BMEC P3 frozen vial was thawed. At P4 cells were seeded and cultured 2…
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hToC | memBrains

Semantic Segmentation for Neutrophils on HUVEC Monolayers: A Machine Learning Approach
ByDan Ahmad November 18, 2020November 18, 2020
Introduction Phase-contrast microscopy is widely utilised in live cell imaging, with pixel segmentation protocols in particular finding widespread usage as a means to computationally isolate cells from a tissue-culture background. Computer segmentation allows for the analysis of individual cell properties such as state or spatial location through automated or semi-automated techniques, ultimately attempting to avoid…
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memBrains | NRG

A Power-free Pneumatic System for Controlling Fluid Flow
ByMeng Hsu November 30, 2022December 5, 2022
Introduction When searching for a flow control system for microfluidic cell culture, we look to balance the ease of use, accessibility, and precision of fluidic control. For instance, researchers have explored different powered actuation methods for miniaturized precise proportional fluid control. These actuation methods include electromechanical, electrostatic, electromagnetic, piezoelectric, and thermal-based. The necessary power sources,…
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memBrains | NRG

Localizing Basement Membrane Production in Coculture BBB Model
ByMolly McCloskey January 29, 2023January 29, 2023
Introduction The basement membrane (BM) is a critical component of a healthy blood-brain barrier (BBB) model. Further, the BM breaks down in several diseases. Both pericytes and endothelial cells can contribute to basement membrane production. This post will explore a method to localize basement membrane components – collagen type IV (Col IV), fibronectin (FN), and…
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memBrains

Utilizing the μSIM to model brain endothelial barrier changes in Cerebral Amyloid Angiopathy
ByMuyu Situ January 24, 2022January 25, 2022
Introduction Vascular hyperpermeability and hemorrhagic stroke are hallmark symptoms of Cerebral Amyloid Angiopathy (CAA), a cerebrovascular disease characterized by amyloid-β deposition around the brain microvasculature. In CAA pathogenesis, vascular Aβ accumulation contributes to structural alterations and loss of integrity in the neurovascular unit/blood-brain barrier (NVU/BBB) resulting in “leakier” blood vessels, intracerebral hemorrhages, increased risk of…
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