memBrains

R61/R33 Update to NIH on Nov. 9, 2022

ByJim November 15, 2022

Attached is a PDF presented to the NIH at our annual update meeting that included other funded project teams.

R61_R33HL154249 11-22 Update (Low Res)

hToC | memBrains | NRG

Merging Man with Machine: A Computer Vision Aided Leukocyte Transmigration Assay
ByDan Ahmad April 25, 2022March 5, 2024
Introduction High throughput imaging techniques in conjunction with in vitro micro-physiological systems (MPS) allow for comprehensive exploration of physiologically relevant phenomena in a living 3D mimetic of a tissue microenvironment. Prior work in this lab has readily demonstrated that vascular tissue mimetics (the µSiM) facilitate real time phase-microscopy visualization of immune cell trafficking in response…
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memBrains | NRG | μSiM

Basement Membrane Disruption by Tumor Necrosis Factor-α in the μSiM-hNVU
ByLouis Widom May 25, 2021June 14, 2021
Note: a postscript regarding an assay to determine the activity of our MMP-2/MMP-9 inhibitor was added to this post on June 14, 2021. Introduction The blood-brain barrier (BBB) plays an important role in maintaining brain chemistry by restricting the permeability of small molecules and cells. Inflammatory diseases may disrupt the BBB and promote the development…
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memBrains | NRG | μSiM

Gene expression analysis of HUVECs cultured on uSiM (static vs flow)
ByMehran Mansouri October 20, 2022November 17, 2022
Introduction Studies have shown that the shear-stimulation of endothelial cells results in the upregulation of the shear-sensitive transcription factor Kruppel-like factor 2 (KLF2). KLF2 plays a critical role in the regulation of endothelial activity such as the regulation of vascular tone, anti-inflammatory responses, and antithrombotic functions. KLF2 is also responsible for the upregulation of endothelial…
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memBrains | NRG

Developing a Distributable µSiM ‘Transwell’-like Platform…Publication Figure Drafts
ByMolly McCloskey November 28, 2021December 1, 2021
Introduction Here, I will post figure drafts for the paper that introduces the modular µSiM (m-µSiM). Figures Remaining data to obtain: Occludin staining in IMR90-4 from UniBe in µSiM. We have tried this after 2 day culture in µSiM, which worked at UR but is not working at UniBe. Current problem: Occludin is not seen in…
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memBrains

hiPSC Differentiation into Brain Pericyte-like Cells, Round 2
ByMolly McCloskey February 22, 2021February 25, 2021
Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and develop strategies that protect against cognitive decline. To fill this gap, we are developing a…
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memBrains

Pump-assisted oscillation mixing in μSIM for consistent small volume sampling and measurement from bottom channel.
ByHoward Su December 20, 2021January 10, 2022
Background From previous experimental results as well as COMSOL simulations presented in our older posts, we observed a universal non-uniform analyte distribution in the μSIM bottom channel due to limited lateral diffusion rate. This uneven distribution profile will limit our sensitivity in using small volumes of supernatants collected from the ports which are far away…
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