Recently, I’ve been frustrated with polycarbonate transwells since they are opaque and I couldn’t see cells on them. So, I bought new transwells from BD (Falcon, PET membrane, 0.4um pores) and decided to test these out. I also wanted to see if coating the transwells with gelatin would promote cell adhesion. These are P6 HUVECs…
I carried out a repetition of BSA coating experiment with both 2D and 3D formats. With 2D, I get the same result as before, but surprisingly, in Sepcon 3D format we observe the opposite to what we see in 2D! (can be seen in the figure below) All the three samples with different BSA coatings…
I coated the pncSi samples from W683 (no RTP) with Bovine Serum Albumin (BSA) in Tris-EDTA buffer. Three different concentrations were used: 1 mg/ml, 5 mg/ml and 10 mg/ml (usual range of BSA conc. used for coating Si surfaces). The samples were left in BSA solution for 45-60 mins at room temperature and almost dried,…
Repeated the previous experiment with W683 and following images were captured after 5 hours of incubation in DMEM(-FBS), Oven @ 37C. I took Sepcon samples with regular thin o-rings, fat o-ring, without o-ring and w/grease in place of o-rings. Also for comparison, used cloning rings and the 2D flat format (left=w/o grease, right=w/grease) samples. All…
I carried out this experiment in order to rule out any effect due to geometry of the setup in our discoloration experiments in Sepcon. I used a cloning ring and mimicked the Sepcon as shown in the figure below. The only difference is the o-ring which is absent in the cloning ring setup. To cover…
Even though we don’t have defect free membranes, I wanted to tweak the gold setup using membranes with pinholes. Previously I was using the small scale diffusion chamber that I use for protein separations. This setup has a retentate of 2-3 uL and a filtrate of 60 uL. To do particle sizing using the Malvern…
We’ve been wondering about the effects of membrane stresses on discoloration rates. This was spurred by Vlassak’s visit and his ideas about stress and membrane stability. In this experiment, I used w501, no RTP, in the oven in RPMI+10%FBS. All of these samples were in the Sepcon format: chip sealed with a fat O-ring, chip…
Last week, I repeated Anant’s experiment which aimed to eliminate edge effects by painting over chips with nailpolish. I painted the entire well side of the chip but tried to keep the wells clear of polish (“painted back”) and I covered just the edge of a chip but tried to keep polish off the well…
I got a couple of wafers from Chris last week – 50nm oxide deposited at room temperature or 450 degrees. For each wafer, I saved the zsensor (top), phase (middle) and zsensor (bottom) scans. I also calculated the roughness (the table looking thing on the bottom of each image. I had some problems with imaging…
First off – OK now on to the science. Wafer 747 has a huge amount of pinholes, and in this study I wanted to see how these pinholes would effect electroosmosis. I will show results here from two experiments with these holey membranes. These membranes have a lower rate of electroosmosis than previous tested membranes. …