I wanted to do some more fluroescent imaging in transwells to look into background fluroescence. This was mainly motivated by my inital study. These are HUVECs stained with 5uM CMFDA and 10uM Hoechst 33342, fixed in formaldehyde and imaged on the Zeiss at 10X. This was 6 hours after seeding. Before acquiring the images, I…
I did an experiment earlier this week in the Sepcon and flat format. I intentionally broke membrane windows so that I could compare chips with no intact membranes vs. chips with 1 intact membrane (and also the effect of autoclaving). Unfortunately, all of the flat chips discolored overnight, so I couldn’t see any differences between…
Last week we attempted to mimic our standard CVC recipe in the AJA system (15 nm Si, 450 C deposition temp, 1000 C RTP). I should note that the protective oxide thickness in the AJA membrane was 20 nm (not 40 nm as it was in the CVC). Below are two images comparing the AJA…
In an effort to imitate Sepcon, I applied nail polish on two samples covering the edges and the back side (well side) and compared its discoloration to Sepcon format and w/o polish sample in 2D format. I used two polished samples in which I covered the edges in both but the back side was not…
We know: W683 has no visible pores, W685 has usual porosity, and the recent Wafer 747 is ridden with pinholes. I wanted to test this new wafer for discoloration in comparison to the other two and got the expected result. In the figure below, there are two sets of images of same samples taken at…
The image below was taken with UR’s new S/TEM, the FEI Technai F20. It has the capability of resolving lattice images which should benefit the materials development effort. However, we will still rely on Karen’s TEM for most of our imaging since the throughput is higher on that tool.
Again a repeat, with comparison to the 2D format. To me, it looks like its again a repeat of what we saw in the previous trials. The sample with both intact slits has slightly higher discoloration than the others and also the pattern seems to originate from the slits (this is what we are looking…
Again a repeat of experiment and the observations. As seen above, the sample with both intact slits discolors the fastest (in Sepcon w/memb side down, DMEM w/o FBS, Oven @ 37C). Result is same as the previous two trials. One thing that I noticed is that the membrane in ‘one slit intact’ sample remains intact…
In the previous post, I used a compound error bar that took into account both image and operator variability. While that seemed to make things a little better, we were still having a problem with w705. I reprocessed a couple extra images from the TEM gallery, and added the differences to the image variability part…
I wanted to attempt pressurized and diffusion separations of brain extract using the new movable wafers. I tried pressure cell separations of 1:20 BBEC (clarified brain extract) at 2psi for 40 min. The flow rates were better than the standards I was previously using – this could be due to the little bit of glycerol…