Here are some high resolution TEM images from a SiMPore-UIUC collaborator. Chris says: The high resolution stuff looks pretty cool, especially the zoom-in on the 8nm pore. I the most obvious crystal planes are <111> with 0.314 nm spacing. If you look carefully, some 0.192 nm <110> planes and 0.136 nm <100> planes can also…
We have seen before that in petri dishes, flipped samples (well side up) discolor faster as compared to the ‘membrane side up’ samples. This could be due to local environment trapped below the chip. Hence, I used Sepcon format so that the sample is raised above the floor to avoid any trapped solution below it….
I repeated the experiment with samples from #703. Two samples were in a dish with one flipped over. Again, the ‘well side up’ sample (flipped) discolored earlier as seen in the 24 hour time point in the figure below.
The movable cutoff gels were more or less successful, but were too dirty for publication (see knowledge page). I’ve been trying to rerun them so that they look nicer, but the darker stain that I used has brought out a few new observations. The first gel following this paragraph is the new gel. The second…
Previous Post We discussed in meeting having Maryna also calculating the permeabilities for this figure. Here is a figure with the experimental, theoretical permeabilities from Dave’s, Maryna’s, and my processing, and an average of the three theoretical permeabilities. I then took this average and made the following figure. I did omit w312, as the super…
After much anticipation, we have confirmed that pores do form with the AJA deposition system! Below are a series of images of membranes of two different thicknesses that were annealed at several temperatures in the RTP. 1000 C RTP; 40/9/40 stack Amorphous; 40/9/40 stack 1000 C; 40/15/40 stack 800 C; 40/15/40 stack Some immediate observations:…
My last cell post involved pictures of HUVECs after 1 day of growth on commercial transwells and pnc-Si Sepcons. Over the past week, I’ve been growing HUVEC on commercial PC transwells and pnc-Si in the Sepcon format in order to track the TEER. To get more data out of these cells, I decided to do…
I repeated the autoclave experiment from earlier this week, but this time I also used the Sepcon format. I used wafer 662, which was contaminated with large spots. This was done in the oven in RPMI-1640 with FBS (no RTP). I think my 0 hour pictures were accidentally erased from the camera, but I think…
I am not sure what prompted me to carry out this experiment. I took few samples from W703 (No RTP) and put them in petri dishes with DMEM (-FBS) at room temperature. I had two samples in a dish, with one flipped over (well side up). The sample with well side up discolored much earlier…
We have seen that discoloration of chips is slower in Sepcons and this has been speculated to be because of the separation between the ‘well side’ and the ‘membrane side’ of the chip existing in the Sepcon format. To further provide evidence to this theory, I carried out an experiment monitoring the discoloration of samples…