Here is the current method that I am following to find the pore statistics: 1. Select the highest threshold that you can still close with disk of 3 2. Hit majority 3. Close w/ 3 4. — Try open w/ 3 5. Erode w/ 3 6. Dilate w/2 7. Dilate w/ 2 8. Erode w/…
W705 performed well in terms of permeability and separation of Hybrid Silica Nanoparticles. No water passed in the wet/dry format (no matter ozone or testing configuration – centrifugal or pressure cell). Reasonably high flow rates when tested in wet/wet configuration in the centrifuge. PVP enabled flow in the pressure cell in an otherwise wet/dry configuration….
We’ve been focusing on bicarbonate ions as one of the causes of discoloration. So, I made several sodium bicarbonate solutions and tracked discoloration of non-RTP’ed samples. I made 3.7 mg/mL NaHCO3 (=44mM, this is the concentration in DMEM), 1.85 mg/mL NaHCO3, 0.925 mg/mL NaHCO3, 0.4625 mg/mL NaHCO3 and 0.23125 mg/mL NaHCO3. I also ordered a…
The last couple of posts showed that homemade HBSS does not cause discoloration, even though commercial HBSS does. I wanted to test if there was something mysteriously special about this solution, so I looked at non-RTP’ed wafer 622 in the incubator in solutions with and without serum (10% FBS). Even without serum, the cell media…
This is a retrial of the negative adsorption assay using the Tecan spec/fluorimeter that we have been demo-ing. To perform this assay, I am applying a known concentration to the surface I want to test. After an hour I remove some of the solution and measuring the remaining concentration using absorption and the Bradford assay….
Demos of the Molecular Devices M2e and Tecan Infinite M200 are done. Erik from Tecan left us the M200 to use until October 6th. Please come try out your assays on it. Or check sensitivities. Jess or I could probably steer you in the right direction as far as the software is concerned. My impressions…
I attached a presentation here for tomorrow’s meeting. sensor
I decided to find another supplier for HBSS and found one: CellGro. CellGro HBSS had the same components as Gibco HBSS, although a couple of the concentrations were different. I tested out this new HBSS vs. homemade HBSS. Here, the commercial versions of HBSS caused discoloration within a day, but my homemade HBSS didn’t. The…
Last week, we wondered whether the wells of pncSi chips created an unstirred volume in which an extreme environment could form (pH, byproducts), thus accelerating discoloration. To investigate this, I placed one chip flat on the bottom of a plate and another chip balanced upright on the sidewall of a well. I rotated the plate…
I sealed track etched membranes into the usual E-Cell setup. Here’s a chart with the calculated flow rates: I’m not sure why the 50nm sample was so much higher. Maybe there’s a difference in porosity? Also this is only the first try at this experiment. Here’s a chart with flow rates of all the experiments…