Intro/Motivation: SiR-Hoechst is a far-red DNA stain for live-cell micro/nanoscopy and has been shown to be non-cytotoxic. I mentioned to Tom that if we can label the cells we can better automate the cell tracking for faster analysis. Tom knew of this dye and decided to give it a try. This direction subsequently lead to a potential…
We are working on finishing up a manuscript on our nanosphere lithography work. this post shows some of the figures for said publication. This paper includes work done with 300 and 200 nm NS as preliminary work, and then focuses on 200 nm NS only to evaluate the porous pattern obtained. From the data presented,…
Hello Everyone, Following of making figures for our first exosome paper, I needed to have SEM images after releasing step. So i ran another experiment and checked the membrane after releasing step. You can see the SEM images of this step: As you’ve probably noticed, compared to after capturing step, you see less captured…
Introduction Over the past two decades there has been little to no improvement in treatment for people who have end stage renal disease (ESRD). The two main methods of treatment for ESRD are kidney transplant or dialysis. However, due to the limitations involved with transplantation, the majority of patients receive dialysis. It has been speculated…
Hello Everyone, Following up the discussion that we had in my last post https://trace-bmps.org/sem-images-for-micro-nano-scale-experiments/about my SEM images after capturing and cleaning. It was suggested to tilt the samples and make sure that beads are inside the pores. So I did a second round of SEM imaging on the same sample. And tilted images clearly…
Introduction When concerning the issue of adherence on any substrate, the main two interactions that matter most are that of the cell interacting with the basement membrane (whether cell produced or artificially added) or the interaction between the protein based basement membrane and the substrate providing structure for cell culture; in our case SiO2 membranes….
1-5-2018 Final tRNA Presentation – By Jon Flax (Beckham Group) Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression
Presentation: Madejski Proposal Presentation v3 Compressed Document: Madejski Silicon Nanomembrane Microsystem Proposal Final Passed in August 2015. Document was criticized heavily, oral presentation was thought to be good.
Hi All, These are documents associated with my proposal: Silicon Nanomembranes for Rapid Liquid Biopsies from Whole Blood and Urine. Proposal: Kilean_Lucas_PhD_Thesis_Proposal_Reduced File Presentation: Proposal Presentation_03
Hello Everyone, I have been trying to take SEM images in 3 different steps during my experiments with polystyrene beads over Track Etched membranes including before capturing, After capturing and releasing. Micro Scale Experiments: As you might remember, in Micro-scale experiments, we use 10 micron beads and 8 micron pores. Before capturing: This is only…