We had previously tested different surfactants to see if we could get 20 nm polystyrene beads and IgG to stay in suspension while filtering with the centrifuge. We found 0.1% Tween 20 to be sufficient when dealing with 10:1 (5×10^14) 20 nm NPs and 1mg/ml IgG. During the reverse centrifuge studies we mixed 10^15 particles/ml…
We have previously tested the effects on filtrate volume after a given time as a function of concentration. Moving forward we want to quantify the improvement in filtrate volume when using reverse centrifugation instead of forward centrifugation. Initially, we attempted to create similar plots to those in the preceding post but got the following gobbledeegook:…
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Bhimani(Ward)10NDT This is a useful paper for our work on Hemodialysis. The authors examine the clearance rates of urea, B2 microglobulin and phosphate as a function of dialysate flow rate . Two things surprise me in this paper: 1) the clearance levels achieved are much higher than I’ve come to expect, and 2) the clearance…
I have been exploring the efficacy of different methods for removing the native oxide from the surface of the sacrificial silicon layer prior to liftoff etch with XeF2. As a reminder, with liftoff of SiN membranes, one can simply dip the sample in BOE solution for a few seconds to strip the native oxide. This…
MAKING CHIPS We now have our own SU-8 mold (with the help of Brian & Alex at URnano) and have started producing chips (see below) with our channel designs. Together with the chips produced last week, we have accomplish one of our objectives: To build a collection of chips for classroom use. With the acquisition…
This paper (SiN paper) by Ulrich Rant et. Al. details several experiments conducted with a single pore drilled through a SiN membrane via TEM. They measure the relationship of pH vs. zetapotential for both a model protein (avidin) and their membrane, and then see how protein moves across the membrane at different pH’s. Translocation is…
Final-Program-MMD-2014.prog_ June 19 Random Notes • Blood Stasis is the main cause of thrombosis • 23 French can access the venous system • 3 French per mm • Extracorporal Membrane Oxygenator used in the treatment of Cardiogenic Shock ◦ Use ECMO as a short term mechanical assist device ◦ Peripheral vs. Central venus access –…
Glavan et al 2013 Lab on a Chip This paper from the Whitesides group details an interesting way to fabricate paper-based microfluidics. They treat ordinary paper with a fluorinated silanization process to render it hydrophobic, and use a Silhouette cutter to scratch a channel into the paper. When covered with double-sided scotch tape, conduits are…
This paper was found when looking for details on quantifying cell spreading and adhesion. The paper discusses how the group has made mesoporous silicon wafers and their success with growing human umbilical vein endothelial cells on the wafers without additional proteins. DifferentialCellAdhesion