Previously, I had gotten some inexplicable results showing that increasing salt concentration decreases the flow through a membrane. This makes no sense (and goes against our previous data) because we would expect electrostatic forces to counteract the flow, an effect called counterelectroosmosis by Zydney: “The situation in the presence of a net fluid flow through…

As the device animation shows, the layout of the chip has changed. The incorporation of through-vias in the bulk silicon allows me to eliminate a gasket wrapping around the filter, which was a large source of leaks. I used ozone bonding for 8 minutes for each layer to join the device together. I initially taped…

              130327 Quote for McGrath Lab – U of R

In order to address the fluctuations in the clearance data at the beginning of the long term treatment previous post The new protocol is as follows: 1. An inspected single slot dialysis chip is clamped into an  acrylic fixture holding PDMS ports with glass capillaries. 2. Ethanol is drawn into the device and the connecting…

BBB Device V2-8 Filling Up     by the way, the code to embed .mp4 videos uploaded to the server is: ” <video width=”640″ height=”360″ controls>   <source src=”http://example.com/wp-content/uploads/video.mp4″ type=’video/mp4′></source>   <source src=”http://example.com/wp-content/uploads/video.ogv” type=’video/ogg’></source> </video>”  

How to use the McGrath Lab Surface Contact Angle Goniometer (SCAG) to collect image data for the established uses. Please refer to the SCAG Setup Protocols for assembly instructions. Note that the details of established uses will be covered in a future post.   General Notes Instrument Layout (See Protocols for SCAG Setup for more part…

How to prepare the McGrath Lab Surface Contact Angle Goniometer (SCAG) from a disassembled form. I will create detailed posts on potential uses in the future. GENERAL NOTES Based on the McGrath Lab SCAG as it was on May 16th, 2013 Instrument layout: All bolts and set screws are 1/4-20’s. The angle screws are 10-32’s. Loosely =…

Although the connection of those electrical circuits was always unstable, Greg and I did get some TEER measurement with this set up:   Device A Day 1 Day 2 Day 3 Day 4 Day 5 EVOM 1 -3450Ω* -1000Ω -1200 Ω -1000 Ω / EVOM2 100 Ω -9600 Ω 450 Ω -3 Ω 827Ω Device…

For the first time, we did time-lapse phase microscopy (1 frame / 5 seconds) looking at endothelial cells through the ITO in the static BBB device (device A bottom left well) and measuring the TEER concurrently. Here’s the video, just shy of 15 minutes of recording.  (reporting a low resistance: 5-10 ohms, shorted). TEER Test…

  http://www.medgadget.com/2013/03/sony-backing-organ-on-chip-technology.html