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We grew some endothelial cells on ITO and glass slides to see if there were biocompatibility issues. We also put some of the thermally cured circuit writer ink afterwards to see if they were cytotoxic. Two devices were constructed: one where cells were growing on ITO and another device where the cells were growing on…
video (this version is saved to the server. It’s smaller and blurrier) http://www.youtube.com/watch?v=2JZ8uTWhejs&feature=youtu.be (this version is private – it can only be accessed by people with the exact link – it’s high quality) I modified the bottom basket of a Sepcon by drilling a small hole into it. A Sepcon chip with an average pore size…
http://www.azonano.com/news.aspx?newsID=26865
What I had in my two previous posts described as permeability graphs were in fact total volume passed/ total time passed graphs: What I thought I saw was that cake layer formation was dependent on the filter geometry. There is some justification for such a dependence in the literature – These folks found that spacer…
Tejas found this… A cool article and video on water ‘sticking’ the surface. Notice the difference in advancing and receding angles. http://web.mit.edu/newsoffice/2013/droplet-surface-adhesion-0219.html
I got some experiences in dealing with cells. This is for your reference. Aseptic Technique and Good Cell Culture Practice Sanitize the cabinet using 70% ethanol before commencing work. Sanitize gloves by washing them in 70% ethanol Put all materials and equipment into the cabinet prior to starting work after sanitizing the exterior surfaces with…
Using methods pretty much identical to those outlined in Tuesday’s post, I was able to get very similar data for a different BSA concentration. Whereas Tuesday’s curves were all done with 1mg/mL BSA, this was done with 0.5mg/mL BSA. The first time I collected sample from the 5 slot chip during the buffer separations, I let…
The following are TECAN spectrum of the filtrates (and a spectrum of unconjugated peptide) for a series of sequential separations performed on the Barcikowski peptide (CE12W) using 10, 20, and 30nm cutoff Sepcons performed last weekend. Note that all of this data was taken with the quartz cuvette not inserted fully into the TECAN as…