At some point, when things settle down, I’ll create a page dedicated to the TECAN machine and document all of the troubleshooting and tricks I’ve learned so far. Before that happens I have another mystery to solve. Take a look at the following three scans: This first are some scans of BSA filtrates from…
After a few more revisions of the micro pump, I performed a significantly more thorough characterization. Flow rate was determined as before using a horizontal capillary of known volume and a stop watch. A extremely small thermocouple was inserted into the PDMS substrate directly adjacent to the fluid outlet channel emptying the…
These volume measures were conducted at 5.6 +/-0.2 PSI using the variable area sepcons with either a half slot, one slot, three slots, or the normal five slots. Note that the pressure was normalized via a pressure regulator, and this normalization was confirmed with a manometer. Filtrates were collected in a series of the…
Updates: We can now put the glass on top of the mask, which allows the use of nonstick agents in the creation of PEG microarrays. The ability to cross-link PEG-Diacrylate (PEG-DA) with a photoinitator and UV exposure was previously tested and shown to work. At the lowest power setting on the Leica (~25%) fluorescence source, we were…
Yuchen and I have been tasked with integrating TEER electrodes into the Cytovu format. The general idea was to fabricate the TEER electrodes similarly to what I have done already, then add them to the current Cytovu device, using UV ozone bonding and PDMS (to cement in the top electrode). I drew some electrodes to…
On Friday Josh and I assembled Sepcon chips with calculated porosity of ~6.5 %, and avg. pore diameter of ~40 nm into Sepcons. I was able to pass 40nm gold through the pores (we don’t have any gold larger than that). Note the “Bad” retentate – for some reason one of the sepcons didn’t filter anything. On…
Please fill out this Lab Specific training form, sign and return to me. If the item is not applicable to your work, sign N/A. Jim LabSpecificTraining(2013).pdf
Fabricate ITO Electrodes Deposit 2500 Angstroms, ITO, Room Temperature Using Kurt Lesker Sputterer (Use ITO Variable Recipe, takes about 1.5hr) Tape in Microscope Slides and Coverglass into Machined Shadow Mask using Kapton Tape Anneal in Toaster Oven, 450 Fahrenheit, 1hr Drops resistance from > 40 Megaohm to < 5 kohm. Measure from edge of Pad…
http://phys.org/news/2013-03-temp-controlled-nanopores-blood-analysis.html
EDIT: This problem was solved thanks to Tejas. It turns out that Falcon BD transparent 96 well plates absorb in the UV range, and are best used for bradford assays or anything that measures absorbance higher than the UV range. Apparently they sell UV-inactive 96 well plates, but they are very expensive. Josh Winans and…