Elder Lab Operation Clean Glass Materials Biological Safety Cabinet (BSC) Ultrasonicator P250 T 0.5 or 1 L glass Beaker ddH2O (Barnstead 18 MΩ) 100% isopropanol Alconox Cleaning Solution Glassware, e.g., Petri Dishes Aluminum Foil Oven that can reach 250°C Gloves (nitrile) 100% Cotton Lab Coat Preparation Put on cotton lab coat and nitrile…
This post is the TraCe and NRG network, letting you guys know that the 5 μm Dual Scale is in stock. They can be checked out by following this link to order from the TraCe-bMPS website and this link to order them from the NRG site. Below are some SEM images of the 5 μm…
This post is about how I prevent contamination in the lab during experiments and material preparation. Contamination has been an issue in sample preparation despite efforts being made to minimize contamination. Current efforts include wearing cotton lab coats across all projects and the MMC, triple rinses with tap water followed by triple MilliQ water, working…
This post is to go over how to post a blog post. This post is written like a protocol, but you can do anything! It is meant to enable live data uploading and review by others. Step 1: Log in to the nanomembranes.org website (or trace-bmps.org for TraCe users). If you are reading this blog…
Introduction The LOMP Small Particle Taskforce is focused on developing a reliable and rigorous procedure for analyzing small microplastics in environmental samples. Our primary aim is to ensure that the data we generate accurately reflects the true microplastic population present in each sample. Achieving this requires careful attention to representativeness, verification, and contamination control throughout…
Introduction The LOMP Small Particle Taskforce is focused on developing a reliable and rigorous procedure for analyzing small microplastics in environmental samples. Our primary aim is to ensure that the data we generate accurately reflects the true microplastic population present in each sample. Achieving this requires careful attention to representativeness, verification, and contamination control throughout…
Introduction The LOMP Small Particle Taskforce is focused on developing a reliable and rigorous procedure for analyzing small microplastics in environmental samples. Our primary aim is to ensure that the data we generate accurately reflects the true microplastic population present in each sample. Achieving this requires careful attention to representativeness, verification, and contamination control throughout…
Administrative: Molly Van Wyk is no longer TraCe-bMPS admin. Assumed Bonnie’s job as Sr. Admin in BME. Will continue to help until position is replaced. Quality Systems meetings are moving to Wednesdays only: 9:30 -10:00 AM Progress meetings will be on Tuesdays 9:00 – 10:00 AM starting in Spring. One more 4 PM meeting planned…
We’ve long speculated that adding fluid flow over our tissue models would reduce the concentration of pro-inflammatory factors secreted in response to inflammatory challenges, dampening the overall inflammatory state compared to static culture. We now have evidence that this is true and the results are important as benchmarks for our future studies. In response to…
Introduction Over the past few months, I have been characterizing and comparing iPSC-derived monocytes (iMonocytes) with peripheral blood-derived (PB) monocytes. As I wrap up these experiments, we’ve decided to look at marker expression on iMonocytes and PB monocytes once they have been polarized to macrophages by culturing the iMonocytes or PB monocytes with HTEN051 fibroblasts…