[Background read – https://trace-bmps.org/blog/theory/2015/01/28/why-teer-values-are-multiplied-by-membrane-area-dc-version] I am putting the excerpt of this old post from Jim in this post. Tom helped me in gathering this data. In order to prove my point that electrical resistance is very sensitive to the membrane geometry, I decided to perform some proof-of-concept experiments with regular transwells. I used 3…
When Aslan and I went to the Beckham lab last week, they gave us a copy of their ultracentrifugation protocol for isolating exosomes. I attached it here so that we can have it as a reference. The major thing to note is that they typically use 13-15 mL volumes of media to begin their centrifugation…
YAP/TAZ YAP/TAZ intracellular localization, and subsequent transcriptional activity, has indicated its role in pathways of mechanotransduction. Investigation of the effects of substrate stiffness on MSC growth and differentiation point to YAP/TAZ as an indicator of how cells are perceiving their mechanical microenvironment and a regulator of the cells’ response to this environment. MSCs cultured on stiff…
Hello Everyone, Here is an update of our meeting in Strong Hospital: So They are working in an urology lab, We met Sam who is a technician in Dr. Carla Beckham’s lab, he is responsible for cell culture part of the project. The cell line that they are working with is T24 with 80 -95% confluency….
SEM of lot # 1208, four-slot 75-nm NPN at 1014x, 1972x, 4101, and 5351x mag. The next image is with a tilted sample. (from the same lot of chips #1208 4-slot 5.4 mm chips) The next five show the edge from the top side. I think the second image is the best of the bunch….
Using the techniques described in my previous post: TEM Tomography of NPN and npMgF2, we have recreated some tomograms that have contour (position) and height (depth) information contained in them. Our goal now is to express that height information in the form of a surface, or volume. Segmentation Having some evidence that…
For the modeling paper, I had to precisely quantify the distribution of gold nanoparticles in the BBI solutions. To do this, I dried the stock solutions of particles on TEM grids (I don’t know what chips I used, but I seem to recall – and take this with a grain of salt – that the grids…
Here are some more images from my ongoing cell culture project. My previous work and protocols: ZO-1 staining of confluent RPE cells on various MgF2 substrates I added twice the amount of primary antibody on these devices that I usually do (1:100 dilution, instead of 1:200). High Porosity MgF2 TCP MgF2 Coverglass
[presentation] HUVEC migration and fibronectin
I will keep this post short and start by saying that the process of establishing a reliable set-up for nanosphere deposition, by evaluating the repeatability of the deposition parameters, has been longer than anticipated. The nanospheres self-assemble at the hexane-water interface and stabilize laterally through electrostatic forces. The main deposition steps are: Add water to…