Objective Develop an easily mainpulated reproducible vasculogenesis assay using hydrogels laden with endothelial cells (‘bio-ink’) patterned using the technique of extrusion bioprinting Bio-inks Develop a bio-ink with the following characteristics Cell viable: bio-ink must sustain cell life, allowing cells to manipulate and move through the hydrogel Vasculogenic: bio-ink must encourage (or at least not negatively affect) tubule formation…
As part of my work over at the University of Nottingham, I’ve been using iBidi devices to grow cells. Eventually, I will use their product to do Raman studies of cells grown on my MgF2 substrates. I’ve been making some progress towards that end using PDMS (I’ve been using 10:1 base to curing agent for…
Hey all! In this post, I’d like to present some of my final data on the exosome isolation project and cover some data that was generated in response to questions asked during our previous discussion. To begin with, Tom asked whether or not it was possible to measure protein in the filtrate and retentate and…
Presentation in this post is based on the paper entitled “Large Area Synthesis of a Nanoporous Two-Dimensional Polymer at the Air/Water Interface”. Paper: Murray(King)JACS15 Supporting Information: Murray(King)JACS15 – SI Presentation Slides (PDF): Two-Dimensional Porous Membranes
160112 LO Grant Update
01-12-16 NSF STTR Meeting Slides in .pptx format
Hi all, I’m Kilean. I’m a first year grad student in BME doing my rotations. My rotation project has been working on developing a method that can isolate exosomes from blood plasma. We are trying to compete with techniques such as ultracentrifugation and ExoQuick, which are both the current preferred methods for isolating exosomes. …
(UPDATED with n=4) I’ve repeated the burst pressure experiments performed by Sabrina, with the 75 nm thick hemodialysis chips. The previous chips are 50 nm with a burst pressure of 1.5 psi (10.5 kPa). I found the burst pressure of the 75 nm chips to be 3.62 psi (25 kPa) n=4. Increasing the thickness 1.5 x lead…
In preparation for the ICNMM conference next summer (and more immediately, the paper they’ve requested by January) I’ve started thinking about what our membranes can offer extracorporeal membrane oxygenation, or ECMO. This post will begin with some background information that I think is particularly important and progress into what work I’ve done so far on…
Introduction: After meeting with Tucker, he showed me the experiment he conducted to induce membrane transfer to, supposedly, any kind of surface. The method he used was initially with breath, and then he passed on to deionized water vapor. The results were not satisfactory because of the wrinkles on the membrane and not much uniformity…