Nanoporous tent wetting fluorescence inconclusive
Due to blog troubles yesterday, we have chosen to upload this post as a .pdf: Nanoporoustentwettingfluorescenceinconclusive
-
-
Vapor Condensation Inside a Nanocavity
My recent work has focused on wetting the inner portion of an attocavity with isobaric cooling to create condensation. We are trying to attack the problem of wetting through the nanomembrane without breaking the membrane. A perfectly dry membrane will always rupture before the critical radius for wetting the backside of the membrane is achieved. To…
-
Analysis of Barrett’s old TEER data
This blog is to revisit the old TEER data obtained by Barrett from the coculture experiments on pncSi and polymer membranes. The reason behind this analysis is to understand the differences of the raw resistance values obtained for the same cell type but on different membrane configurations. The data is shown below. As we can…
-
Wetting of Nanocavities using Isobaric Cooling
Summary and Instructional video The simplest way to get something wet is to allow water to condense on the surface of the something. In our case, we have a number of nanocavities (~ 0.1 femtoliters) with porous tents over them. The easiest thing to do is simply chill the substrates down to allow condensation to form….
-
Smooth and Suspended Nanomembrane Tents
I wrote about my experiences tenting nanomembranes here. I delaminated NPN material across a bunch of substrates and found that the film would actually form a nanocavity if spread across a sufficiently small surface (I estimated it to be 1-2 microns over a 50 nm height difference). Simpore fabricated a number of small substrates with varying…
-
Nanoporous SiN Lift-Off Update – April 2015
Background Details: Josh Miller & I have made some recent progress in the NPN LO work. We had several ø6″ wafers w/ 100 nm of thermal oxide processed by Rogue Valley, wherein they deposited 650 nm of poly-Si (LP-CDV) followed by 50 nm of low-stress SiN (LP-CVD). Josh added the a-Si + SiO2 layers at…
-
Fouling Mechanism in Josh’s 20 nm, 100 nm SepCon data
These fits are done using the math outlined in this previous post. We assume an average pore size of 50 nm and an approximate number of pores in the membrane of 1E9. 20 nm particles: 100 nm particles: Next, we were curious whether we could show the transition happen from pore constriction to…
-
Magnesium Fluoride Burst Pressure Wafer #2
I was not entirely satisfied with the amount of data that I received from my last burst pressure wafer, so I processed another wafer. This wafer had some yield loss initially, as well as window sizes that were slightly smaller than expected (by about 50 microns). We expect the mechanical strength of the windows to…
-
Derivation of an Analytic Solution to the Problem of Flux Decline and Fouling in a Centrifuge Tube
In my previous post, I derived a formula for the time-dependent flux decline in a centrifuge-driven membrane separation, assuming that the resistance of the membrane was held constant. The problem with that approach is that, as Josh discusses in this post, the resistance of the membrane increases with time according to one of several standard…
-
Heart on a Chip (Healy Group)
Kevin Healy is a Phase I and Phase II awardee of the NIH Tissue Chip initiative. The goal of this initiative is to create ‘mini-organs’ ‘organs-on-a-chip’ ’tissue chips’ or ‘microphysiological systems’ (MPS) for higher throughput and more reliable drug discovery. The effort is also motivated by the failure of recent animal models to predict drug…