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AFM of SiO2 film roughness
Dave did a series of 20nm SiO2 film depositions at different temperatures (constant bias) and gave them to me to AFM. I guess he wanted to see if film roughness changed drastically over the range of ‘room temp – 400C’ in order to investigate this as a potential source of pinholes. I used a new…
Discoloration Study with Bacterial Media
I performed a test study to see the effect of microbial media’s : LB and BHI Broth on the membrane to check for discoloration. LB Broth : Lysogeny broth,Luria broth or Luria-Bertani broth. Obtained from Sigma and made by dissolving 20g in 1 l of D.H2O and autoclaving at 121C for 15 mins. LB is…
More vacuoles on free-standing than supported pnc-Si (and PET)
One of the other suggestions from Angela Glading at lab meeting a while ago was to quantify the vacuoles on different culture areas. So, I selected images from 3 different experiments (all at day14 of growth) and counted the number of cells which expressed vacuoles on different areas. I then used my mask code in…
Manual Reconstruction of Electron Tomograms
Using the techniques described in my previous post: TEM Tomography of NPN and npMgF2, we have recreated some tomograms that have contour (position) and height (depth) information contained in them. Our goal now is to express that height information in the form of a surface, or volume. Segmentation Having some evidence that…
No Time To Waste: Developing a Faster Method for Viral Genome Extraction from Waste Water Samples
The premise of this project is built around the ideas that our membranes can: Be used as concentrators for very dilute targets Catch and hold particles for further processing on the membrane Here, we wish to test these ideas for dilute viral genome extraction in large volumes of waste water. We looked to improve upon…
Post-etch annealed membranes
Below are images from two samples that were annealed at 950 C and 1050 C post-EDP etch. The lower temp anneal produced a membrane with many features that do not seem to penetrate the nc-Si. There were a few (couple in each field) smaller pores ~ 10 nm in the outer samples. This supports Jess…
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Maryna,
Can you post a version of this figure with controls and the carbon nanotube and track etched data from the papers we’ve reviewed lately. I understand that these will be near zero and not very legible on the scale for our membranes. Thats OK.
Thanks.