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So vapor salinization slows degradation but does not prevent it and L15 is just fine.
1) We’ll need to find more complete surface chemistry to work in general media.
2) We still need to do the ‘reconstitution’ studies to prove that it is a combination of bicarbonate and salts that causes the trouble in DMEM and other media – lets do this next please.
Anant,
If you vapor silane treat the surfaces – but don’t oxidize them beforehand with peroxide – what do you think is happening?