MgF2 + Si3N4 Nanomembrane Images

Hi all, Recently we finished drafting the manuscript titled “Porous substrates promote early endothelial migration at the expense of fibronectin fibrillogenesis.” This manuscript will feature the cover illustrated by Brad (our new medical illustrator in the Gaborski Lab):   Long story short (another illustration by Brad): Left (nonporous control substrate): cell traveled slower, covered smaller…

Using Mike B.’s MATLAB script, I was able to generate some histograms and stats for wafer 187, 310, 319 and 320. I excluded the “small pore background”, as I do not believe these features go all the way through the membrane. wafer 187 wafer 310 wafer 319 wafer 320 The reason why I did not…

The goal of the design is to design a system that can co-culture cells under varying flow conditions, measure TEER, and perform dynamic microscopy. Specifically, the endothelial tight junctions are the primary mechanism by which the BBB is regulated. It is thought that astrocytes and pericytes can influence this mechanism. With the completed system, we…

Last week, five wafers were produced – three had a double 3500 A wet oxide growth/strip and two had a single 3500 A wet oxide growth/strip.  From Jess’ AFM work, an as-is wafer showed a high density of 5-7 nm particles on the wafer surface.  With a double oxide growth/strip, these particles do not show…

5nm BSA-Au appears to be 12.7nm.10nm BSA-Au is 18nm. Dilution dependent size change of 5nm BSA-Au. One theory for the size change was that residual BSA was combining with the Au peak and for some reason dilution of the BSA was causing a migration of the peak. This doesn’t necessarily make much sense as the…

We grew some endothelial cells on ITO and glass slides to see if there were biocompatibility issues. We also put some of the thermally cured circuit writer ink afterwards to see if they were cytotoxic. Two devices were constructed: one where cells were growing on ITO and another device where the cells were growing on…