Flow demo (T cell interaction with human in vitro BBB model under flow)
I attached a link to a short presentation regarding the flow movie setups. The figures and videos will be explained in detail during the meeting on Friday.
Similar Posts
Collagen IV deposition by hCMEC/D3 (endothelial cell) and HBVP (pericyte) co-cultures in the μSiM-hNVU
Note: A postscript regarding a gelatin assay to determine MMP-9 activity was added to this post on Feb 25, 2021. Introduction The blood-brain barrier (BBB) plays an important role in maintaining brain chemistry by restricting the permeability of undesirable small molecules and cells. Inflammatory diseases may disrupt the BBB and promote the development of neurological…
Fibronectin and Friends: Confirming the Presence of Multiple Vascular Basement Membrane Components in the μSiM-hNVU
Introduction The blood-brain barrier (BBB) plays an important role in maintaining brain chemistry by restricting the permeability of small molecules and cells. Inflammatory diseases may disrupt the BBB and promote the development of neurological disorders [1]. One potential mechanism of BBB disruption is the degradation of the extracellular matrix proteins that make up the vascular…
2 Comments
Comments are closed.
Micro three-way valve http://www.theleeco.com
#LFYA1226032H 12volts 0.054inch ports(push on tubing)& deadspace=22uL
You do need to make an electronic box- I had one made by our department’s electronics guy.
Thanks for showing us your system- Always interested in seeing the clever ways people do this stuff!
Coisne, C., R. Lyck, and B. Engelhardt, Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo. Fluids Barriers CNS, 2013. 10(1): p. 7.
I upload pdf file of this paper and recorded zoom meeting in media.