Flow demo (T cell interaction with human in vitro BBB model under flow)

Introduction Pericytes are mural cells that line the outer surface of microvessels and regulate diverse aspects of vascular development and function. We differentiate brain-pericyte-like cells (BPLCs) from hiPSC clones using the protocol published by Eric Shusta’s lab (Gastfriend et al., 2021) (Figure 1). This protocol describes methods to differentiate hiPSCs to BPLCs via neural crest…

One of the validations of the in vitro human Blood Brain Barrier system is to ensure that the endothelial cells derived from iPSC’s and cultured in our system exhibit behaviors that are consistent with endothelial cell behavior in situ.  The particular aspect of BBB endothelial function addressed in this post is the expression and properties…

1. Introduction Fluid flow is one of the key elements of vascular models for delivering nutrients and growth factors to cells as well as introducing other components like neutrophils. In post 1, we described our modular design for incorporating a flow module into the uSiM device. Then, we presented fluid flow simulation and its experimental…