Flow demo (T cell interaction with human in vitro BBB model under flow)

Introduction We are differentiating and validating EECM-BMEC (Extended Endothelial Cell Culture Method-Brain Microvascular Endothelial Cell)-like cells in µSiM devices. See this post and this post for method details and validation in traditional cell culturing platforms. Methods Attempt 1 – Overstimulated cells IMR90-4 iPSC EECM-BMEC P3 frozen vial was thawed. At P4 cells were seeded and cultured 2…

Introduction In a previous post, an initial version of the flow module and its sealing components were described. In order to improve the initial version based on collaborators’ feedback, several modifications have been made to simplify the device assembly for the end-user (Fig. 1) and address concerns about: 1) Local regions of high shear stress…

Introduction Pages regarding our in situ permeability assay development can be found in these links: Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 1: Approach) Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 2: Experimental Validation) Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 3: Application to Cell…