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AFM – wafer characterization
Chris gave me a few wafers to look at with AFM – an oxide sputtered in the recently cleaned chamber and VA Semiconductor wafers cleaned with “Normal Power” or “Full Power” Megasonics. I employed a couple of new “tricks” to help with the imaging this time. All of these scans were taken almost entirely in…
SU-8 Shrinkage
Goal: Measure SU-8 shrinkage that have gone through various soft and hard bake conditions To measure the shrinkage, the SU-8 scaffolds needed to be lifted off, but there was some issues. Originally, the SU-8 was spincoated onto an oxide layer, which would be etched away using BOE. The SU-8 had appeared to be disintegrating due…
UV-vis Spectrophotometer Measurements on NPN
We’d like to get verification of the absorption spectra of our npAu/npMgF2 SERS structure. The SERS effect should occur where the absorbance is maximized. Total Light = Absorbance + Reflection||Scattering + Transmittance Last Friday, I had the opportunity to work with Jennifer Kruschwitz who possesses a Konica-Minolta integrating sphere spectrophotometer from 380-740 nm (10 nm…
This is probably not of general interest, but for the sake of full disclosure, I have spent considerable time working with our wafer supplier (Virginia Seiconductor) to resolve some issues regarding the surface quality of their wafers. It would have been much more productive to simply switch suppliers, but unfortunately, they are the only place…
Dye Diffuses through UV-Ozone Treated and Untreated SC267 Samples
ByMike HIn my last post I presented the results of an experiment in which it appeared that BSA did not diffuse through a SC267 sample treated in the UV-Ozone system for 20min at 150C. As a control I also ran BSA dissusion through an untreated SC267 sample and showed that BSA did move across the membrane….
Calcein vs. CMFDA staining
The other (see below) just-to-be-sure experiment I performed this week was to compare staining between 2 different live cell dyes. I typically use calcein AM to stain live cells green. This is a membrane-permeant dye which, once inside cells, is hydrolyzed by intracellular esterases to make it fluoresce green. Another very common live cell stain…
