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Nanomembrane Research Group
  • NRG

    Absorbance Problems with Colored Centrifuge Tubes

    BySarah Wayson August 26, 2013

    This is a follow up to Josh’s post, “Continued Au Separations with SiN-NP wafer #’s 1029 and 1030”, and seems to explain why we initially got an S value greater than one during our gold separations. We conducted tests to see if the colored centrifuge tubes were at fault for our seemingly large filtrate absorbance…

    Read More Absorbance Problems with Colored Centrifuge TubesContinue

  • NRG

    FLAG Separation Experiments with an Enzymatic Activity Assay

    ByTucker Burgin August 23, 2013

    As I mentioned in my previous post, there are a lot of problems associated with the technique for testing the ability of a CytoVu assembly to act as an assay that I had been trying to use before. Several possible sources of error were apparent, such as the difficulty inherent in consistently pipetting such small…

    Read More FLAG Separation Experiments with an Enzymatic Activity AssayContinue

  • Knowledge | NRG

    Hemodialysis in the rat animal model

    ByDean Johnson August 22, 2013December 14, 2018

    I found some good descriptions of hemodialysis experiments using rats. They all use a method possibly first described by (Rockel et al., 1978). (Galons, Trouard, Gmitro, & Lien, 1996) at U of Arizona used nephrectomized male Spraque-Dawley rats (Holtzmann Co., Madison, WI) weighing ~300 g to study the effects of hemodialysis on the apparent diffusion…

    Read More Hemodialysis in the rat animal modelContinue

  • NRG

    More in-situ heating videos

    ByJoe Qi August 20, 2013

    Here are some other in-situ heating videos I made recently. First one is NSN30730, which means the Si is 7nm and Si3N4 is 30nm. The speed of the video is 10 time faster than the real time. The first figure is the heating illustration and then comes with the video. The next is sample NSN301030….

    Read More More in-situ heating videosContinue

  • NRG

    Burst Pressure III

    BySarah Wayson August 20, 2013

    This is a follow up to my previous post, “Burst Pressure Probability II”. I burst 111, 30nm thick single window pnc-SI TEM chips of ten different window sizes, to measure the burst pressure of chips with varying dimensions. Although area is a major determining factor in the pressure at which the windows break, two different windows of…

    Read More Burst Pressure IIIContinue

  • NRG

    Hydraulic permeability of SiN-NP wafer #'s 1029 and 1030 with "clean" water

    ByJosh Winans August 20, 2013January 23, 2014

    Sarah, Tejas, and I bought some “Invitrogen Ultrapure DI water” from the medical center to see if we would find a different permeability using the clean water vs the water from the Barnstead. Sarah and I repeated our permeability tests but made sure to measure the flow rate for the first 100 uL.  We got…

    Read More Hydraulic permeability of SiN-NP wafer #'s 1029 and 1030 with "clean" waterContinue

  • NRG

    prediction of flow distribution within a membrane-based shear-free system

    ByHenry August 20, 2013January 20, 2015

    Hi all, Previously we described a two-compartment flow system integrated with a nanoporous membrane in between; and we showed that the nanporous membrane effectively reduces fluid flow from one compartment to the next. Seeing that today is our last NRG meeting this summer, I will present a revisit to the prediction of flow distribution within the membrane-based…

    Read More prediction of flow distribution within a membrane-based shear-free systemContinue

  • NRG

    Karl’s Streaming Potential/EO Device

    ByKarl Smith August 20, 2013January 22, 2015

    I’ve been working on building a streaming potential/EO device for a while now. This was Yaron’s prototype: Which itself was based on Jess’s design: I didn’t like that the device needed to twist together, and also that it was designed for the old circular 2-slot sepcons. My first prototype used the machine vise downstairs for structural…

    Read More Karl’s Streaming Potential/EO DeviceContinue

  • NRG

    Pores in SiO2 layer

    ByJoe Qi August 20, 2013

    Here is an interesting discovery of nanopores in the SiO2 layer after the laser annealing. Basically I was doing laser annealing on free-standing OS membrane the other day. It’s an excimer laser  with 248nm wavelength, 25ns FWHM and spot size of 2.6 mm diameter. Same setup we used before in ORNL. The sample we test…

    Read More Pores in SiO2 layerContinue

  • NRG

    Updated: Continued Au separations with SiN-NP wafer #'s 1029 and 1030

    ByJosh Winans August 20, 2013January 23, 2014

    Sarah and I did some more gold separations with 10, 20, 40, 50, 60, 80 and 100 nm Au particles.  We used the “clean” water purchased from the medical center.  Same as last time, we diluted the “as-bought” gold 3 parts to 1 and loaded each cell with 300 uL before pressurizing at 5 psi…

    Read More Updated: Continued Au separations with SiN-NP wafer #'s 1029 and 1030Continue

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    • Home
    • Publications
    • Membranes
      • Common Chip Formats
      • Common Membranes
      • Microslit Membranes
    • Devices
      • µSiM
        • Geometry
        • µSiM CAD Files
        • Assembly
          • Protocols.io (µSiM Assembly)
          • Instructions
          • Common Issues and Troubleshooting Tips
        • Cell Culture Protocols
          • Top Well: hCMEC/D3
          • Top Well: HUVEC
          • Bottom Channel Culturing
          • Immunocytochemistry Protocol
          • Impact of Chip Orientation on Fluorescence Imaging
          • Permeability: In Situ Method
          • Permeability: Sampling Method
          • Cell Culture Common Issues and Troubleshooting Tips
      • SepCon®
        • Sepcon Assembly
        • Sepcon Video Protocol: Assembly
        • SepCon Gasket Silhouette File
        • SepCon Video Protocol: Wetting the membrane
        • SepCon Video Protocol: Disassembly
      • µSiM-DX
        • µSIM Video Protocol: Capture of Nanoparticles
    • Impact
      • TraCe-bMPS
      • HCIC
      • LOMP
      • SiMPore