Silver chloride electrodes are useful for microfluidic devices since they can generate current below one volt. This allows them to function without fear of electrolysis confounding experimental results by way of gaseous clogging. Several different methods for coating silver mesh were tested to determine which would be ideal for future experimentation. These methods were as…
Presentation from yesterday: PPT, PDF
In this trial, bEnd.3 cells were seeded onto three single-well CytoVu devices with SiO2 membranes coated with a 1% Geltrex coating. After ~27 hours, the cells were fixed and permeabilized (the fixing may be only partial though, as the solution used was more than 5 months old). After this, DAPI and FITC-conjugated phalloidin were used…
Karl and attempted to pass 10, 20, 40 and 50 nm Au NPs through samples from the center of wafer # 1019 (avg por dia 50-60 nm, porosity ~27%). The bottles of stock Au from BBI solutions do not have the concentrations marked, but according to BBI’s website, they only sell the concentrations provided in…
The Gaborski lab has been working with Blanca’s lab performing some scoping tests w/ SiN and SiOx microporous membranes. The aim is obtain proof of concept results regarding the efficacy of using a microporous membrane to trap sub-micron particles with DEP. Last week, we tested w/ our 200 nm polycarbonate beads. We were able to…
We’ve been using gold nanoparticles (available for ~80 for a 25mL bottle from Sigma Aldrich in sizes up to 400 nm) for years in the lab. This post highlights some of the previous work that has been posted to the blog and brings together some of the information in the literature and on supplier’s websites. First, some…
Fluorescent beads were employed to assess electroosmotic flow in various devices. The beads were 200 nm in size and were negatively charged (on account of being carboxylated polystyrene). These beads were suspended in a 0.2 mM K2HPO4 buffer. They were also incubated in 1% BSA solution in an attempt to reduce their surface charge, though…
Today I performed a coating of n-Dodecyl β-D-Maltoside (“DDM”) on PDMS in an attempt to block protein adsorption (reference). The reference describes two different procedures, one in which the protein being incubated on the PDMS surface is in solution with DDM, and one in which the DDM coating is performed before the sample is washed…
Over the past few days Josh and I installed windows 7, windows office suite, Malvern’s Zetasizer software (available online), Tecan’s I-control software (available in the 3-ring binder in the glass cabinet above the machines), and chrome onto the new(er) laptop in the basement. I copied the files on the old computer that seemed relevant –…
Permeance Testing Sepcons were built with 500 nm 5-slot chips. Permeance tests were performed on Samples A-C. Nitrogen gas was pushed through the membrane at a given pressure, and the height on the flow meter tube (green graduated column pictured below) was recorded according to the location of the center of the silver ball. No units…