From Jess’ manuscript … From Wikipedia … From Tom’s ACS nano paper … The Dagan equation (2) for flow through short pores used to predict flow through pnc-Si is given by: where r is the average pore radius, DP is the pressure drop across the membrane, l is the membrane thickness and m is the…
Vaccinex has developed an antibody, VX15/2503, for Semaphorin 4D, a molecule implicated in axonal guidance mediation and blood brain barrier (BBB) disruption. The role of SiMPore in its partnership with Vaccinex is to produce a workable model of the BBB, with the ability to introduce flow conditions and measure an electric gradient across the membrane. …
As a rotation student from the Biophysics depart, I’ll be spending the next six weeks or so helping Dr. Stephan Barcikowski use the nanomembranes to do a number of separations as part of a nanofabrication procedure. Dr. Barcikowski makes extremely pure gold and platinum nanoparticles (~10nm) colloids using laser ablation and then bioconjugates them with…
One of the surprising results of Jiracai’s studies with thick and thin membranes was that the thicker membranes had a lower capacity to support back pressure than the thinner membranes he used (see table in this post). This is counter to our original expectation; Since we know that much thicker EO membranes can support 50…
As discussed in a previous post “Clearance, Kt/V, and Concentration…”, we will use the Kt/V metric to measure the effectiveness of dialysis. There are other methods that are used: one of these is the Urea Reduction Ratio, URR. URR is a fractional decrease in Blood Urea Nitrogen, BUN, in a single dialysis session. It is…
Jirchai summarized his data in this table … A question raised by a reviewer of our PNAS submission – and one that we’ve had for a long time – is what is the efficiency of the pump. The electrical power provided is calculated in the table for each configuration. As Chris mentioned in group meeting,…
Dialysis Rate: 10 mL/min, Péclet Number: 1, Membrane Width: 700 µm, Channels per chip: 17 Péclet number Pe ∝ H Pe ∝ 1/L Pressure Drop, P P ∝ 1/H3 P ∝ L
When we submitted the EO paper way back one of the reviewers made the following comment … What is the “head pressure” P_max, i.e. the maximum back pressure that would stop the pump? For any linear electrokinetic phenomenon such as EO pumping, the flow rate decreases linearly with backpressure until a certain point (“head pressure”)…
The urea assay is straight forward. 6 wells are filled with 0, 1, 2, 3, 4, and 5 nmol of the urea standard (50 nL). Each well is activated with 50 nL of the reaction mix. Reaction Mix Sample control Urea Assay Buffer 44µl 46µl OxiRed Probe 2µl 2µl Enzyme mix 2µl 2µl Developer 2µl…
In my previous post of Morphology evolution in pnc-Si membrane (2), see https://trace-bmps.org/datablog/?p=14769&preview=true , I showed that membranes (30SiN-25Si-30SiN) annealed at 700C show us an intermediate morphology in the pnc-Si membrane. I did some further investigation in the past week on this intermediate morphology. Compared with the previous test which membrane was annealed at 700C…