Overview Recently, we performed a series of flow cytometry studies assessing neutrophil (PMN) activation in first generation flow devices. All the relevant data and can be found in a previous post: here. As part of the analysis, we chose to quantify relative PMN activation by comparing isotype control histograms against L-selectin (CD62L) expression histograms. This…
One of the more useful formulas for our materials is Equation 1 in Chung et al., 2014 Lab on a Chip 14:2456-68 Assuming uniformly distributed pores in a planar (i.e. ultrathin) membrane, the relationship between pore radius r, porosity p, and the average spacing between pores, Lp-p is: Where the porosity is a number between…
The Burden of Tendon Injuries on HealthCare: Acute and chronic tendon injuries commonly affecting the working-age population are frequently work-related and have negative socioeconomic outcomes such as productivity loss, income loss, and unemployment(O’Hara et al., 2020). Tendon injuries are often multifaceted and can be characterized by inflammation and degenerative changes in tendon structures collectively known…
Introduction Vascular hyperpermeability and hemorrhagic stroke are hallmark symptoms of Cerebral Amyloid Angiopathy (CAA), a cerebrovascular disease characterized by amyloid-β deposition around the brain microvasculature. In CAA pathogenesis, vascular Aβ accumulation contributes to structural alterations and loss of integrity in the neurovascular unit/blood-brain barrier (NVU/BBB) resulting in “leakier” blood vessels, intracerebral hemorrhages, increased risk of…
1 m^3 = 1000 L 1 cm^3 = mL 1 mm^3 = uL 1 um^3 = fL 100 µm x 100 µm x 100 µm = 1 nL 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1 aL
The premise of this project is built around the ideas that our membranes can: Be used as concentrators for very dilute targets Catch and hold particles for further processing on the membrane Here, we wish to test these ideas for dilute viral genome extraction in large volumes of waste water. We looked to improve upon…
Thanks to our collaborators at the University of Chicago we have now used NPN to demonstrate sodium/hydrogen exchange functions at the level of single EVs. This is first-of-a-kind evidence of protein function in individual EVs. It will be officially published in Communcations Biology and will soon be located at this DOI. What follows are key…
Slide deck from Large Group Meeting 12-21-12 (PPT) Access requirements for the AFM cantilver to contact the cells requires the cells to be grown on the membrane surface in what is typically the bottom channel of A-line devices (Typical A-line configuration is shown in slide 2). A PDMS block is attached to the well side…
Introduction The blood-brain barrier (BBB) plays an important role in maintaining brain chemistry by restricting the permeability of small molecules and cells. Inflammatory diseases may disrupt the BBB and promote the development of neurological disorders [1]. One potential mechanism of BBB disruption is the degradation of the extracellular matrix proteins that make up the endothelial…
Background From previous experimental results as well as COMSOL simulations presented in our older posts, we observed a universal non-uniform analyte distribution in the μSIM bottom channel due to limited lateral diffusion rate. This uneven distribution profile will limit our sensitivity in using small volumes of supernatants collected from the ports which are far away…